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Mas7 Catalog No.GC36545

Size Price Stock Qty
1mg
$83.00
In stock
5mg
$334.00
In stock
10mg
$519.00
In stock
25mg
$871.00
In stock

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Sample solution is provided at 25 µL, 10mM.

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Protocol

Cell experiment:

Hippocampal neurons cultured in round 35?mm coverslips at a density of 160,000 cells/coverslip are transfected with EGFP at 11 DIV. Then, at 14 DIV the neurons are placed in the imaging chamber in an isotonic solution. The EGFP-positive neurons are imaged with microscope every 5?min for 45?min after the treatment with 1?μM Mas-7. The images are processed and analyzed using ImageJ software[1].

References:

[1]. Bavec A, et al. Novel features of amphiphilic peptide Mas7 in signalling via heterotrimeric G-proteins. J Pept Sci. 2004 Nov;10(11):691-9.
[2]. Grze k G, et al. Direct regulation of vascular smooth muscle contraction by mastoparan-7. Biomed Rep. 2014 Jan;2(1):34-38.
[3]. Ramírez VT, et al. The Gαo Activator Mastoparan-7 Promotes Dendritic Spine Formation in Hippocampal Neurons. Neural Plast. 2016;2016:4258171.

Chemical Properties

Cas No. 145854-59-7 SDF Download SDF
Synonyms N/A
Chemical Name N/A
Canonical SMILES N/A
Formula C67H124N18O15 M.Wt 1421.81
Solubility Soluble in DMSO Storage Store at -20°C
General tips For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.
Shipping Condition Evaluation sample solution : ship with blue ice
All other available size: ship with RT , or blue ice upon request
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Background

Mas7, a structural analogue of mastoparan, is an activator of heterotrimeric Gi proteins and its downstream effectors.

Mas7 produces several biological effects in different cell types. The effect of Mas7 on endogenous mono-ADP-ribosyltransferase activity is in the micromolar range with a maximal activation of 205% over the basal. In pertussis treated plasma membranes, it is found that the effect of Mas7 on endogenous mono-ADP-ribosyltransferase is partially blocked, which suggests the involvement of G-proteins, such as Gi or G0[1]. Mas7 is a basic tetradecapeptide isolated from isp venom, which activates guanine nucleotide-binding regulatory proteins (G-proteins) and stimulates apoptosis. In smooth muscle cells, Mas7 leads to an increase in the perfusion pressure. Vascular contraction is induced by Mas7. The vasoconstriction triggered by mas-7 exhibited a slower increase compared to that simulated by phenylephrine or vasopressin[2]. Exposure of hippocampal neurons to a low dose of Mas-7 increases dendritic spine density and spine head width in a time-dependent manner. Additionally, Mas-7 enhances postsynaptic density protein-95 (PSD-95) clustering in neurites and activates Gαo signaling, increasing the intracellular Ca2+ concentration[3].

[1]. Bavec A, et al. Novel features of amphiphilic peptide Mas7 in signalling via heterotrimeric G-proteins. J Pept Sci. 2004 Nov;10(11):691-9. [2]. Grze•k G, et al. Direct regulation of vascular smooth muscle contraction by mastoparan-7. Biomed Rep. 2014 Jan;2(1):34-38. [3]. RamÍrez VT, et al. The Gαo Activator Mastoparan-7 Promotes Dendritic Spine Formation in Hippocampal Neurons. Neural Plast. 2016;2016:4258171.