Coumarin 6 |
Catalog No.GC38139
One-Click Copy Product Info
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Coumarin 6 is a stable laser dye (λex=450nm, λem=500nm) and is a useful probe in variety of chemical and physicochemical studies.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 38215-36-0
Sample solution is provided at 25 µL, 10mM.
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Coumarin 6 is a stable laser dye (λex=450nm, λem=500nm) and is a useful probe in variety of chemical and physicochemical studies[1]. Coumarin 6 is used as a fluorescent dye for microplastics, is soluble in organic solvents but insoluble in water, Coumarin 6 emits intense green fluorescence when exposed to blue light[2]. Due to the extremely low solubility (with a solubility in water lower than 0.25μg/ml), Coumarin 6 can be regarded as a typical hydrophobic model drug[3]. Coumarin 6 can be encapsulated in single-chain liposomes and administered by eye drops, which is used for retinal flat imaging in animal models[4]. Coumarin 6 can form a complex with β-cyclodextrin, which can be used to quickly distinguish different types of cells[5].
References:
[1] Satpati A K, Kumbhakar M, Maity D K, et al. Photophysical investigations of the solvent polarity effect on the properties of coumarin-6 dye[J]. Chemical physics letters, 2005, 407(1-3): 114-118.
[2] Cheng Y, Zhang J, Nakano H, et al. Coumarin 6 staining method to detect microplastics[J]. Marine Pollution Bulletin, 2023, 193: 115167.
[3] Miao X, Li Y, Wang X, et al. Transport mechanism of coumarin 6 nanocrystals with two particle sizes in MDCKII monolayer and larval zebrafish[J]. ACS Applied Materials & Interfaces, 2016, 8(20): 12620-12630.
[4] Inokuchi Y, Hironaka K, Fujisawa T, et al. Physicochemical properties affecting retinal drug/coumarin-6 delivery from nanocarrier systems via eyedrop administration[J]. Investigative ophthalmology & visual science, 2010, 51(6): 3162-3170.
[5] Edetsberger M, Knapp M, Gaubitzer E, et al. Effective staining of tumor cells by coumarin-6 depends on the stoichiometry of cyclodextrin complex formation[J]. Journal of Inclusion Phenomena and Macrocyclic Chemistry, 2011, 70(3): 327-331.
Staining of tumor cells by Coumarin 6
This protocol only provides a guideline, and should be modified according to your specific needs
1. Stock and dilute solution preparation for Coumarin 6 staining of cells
(1) A 60μM Coumarin 6 stock solution was obtained by dissolving Coumarin 6 powder in absolute ethanol resulting in a clear, green fluorescent solution. Dilute the Coumarin 6 stock solution (60μM) with absolute ethanol to a working solution with a final concentration of 1μM.
NOTE: The working solution must be prepared fresh on the day of the experiment.
(2) Complex formation between Coumarin 6 and β-cyclodextrin
Ethanolic Coumarin 6 (working solution) and aqueous β-cyclodextrin was mixed in varying amounts to obtain the desired Coumarin 6/β-cyclodextrin concentration ratios. The mixture was stirred for 10min at room temperature and this procedure gave a clear, highly green fluorescent solution in the case the β-cyclodextrin concentration exceeded 20μM. To stain cells the solution was added to the growing buffer for a final concentration of 30nM for the dye.
2. Cell staining and epifluorescent images
(1) Bladder cancer cells were grown in chambered cover slides in MEM medium at 37°C and 5% CO2.
(2) For imaging the medium was replaced by phosphate buffered saline (137mM NaCl, 2.7mM KCl, 10mM Na2HPO4, 1.76mM KH2PO4, pH 7.4) containing the Coumarin 6/β-cyclodextrin complex at a final concentration of 30nM for Coumarin 6.
(3) Epifluorescence images were taken after 10min incubation, appropriate filter settings and ×10 magnifications.
3. Experimental Precautions
(1) Coumarin 6 solution should be stored in the dark throughout the experiment.
(2) This protocol and all reagents are for scientific research use only.
References:
[1] Edetsberger M, Knapp M, Gaubitzer E, et al. Effective staining of tumor cells by coumarin-6 depends on the stoichiometry of cyclodextrin complex formation[J]. Journal of Inclusion Phenomena and Macrocyclic Chemistry, 2011, 70(3): 327-331.
| Cas No. | 38215-36-0 | SDF | |
| Canonical SMILES | O=C1C(C2=NC3=CC=CC=C3S2)=CC4=CC=C(N(CC)CC)C=C4O1 | ||
| Formula | C20H18N2O2S | M.Wt | 350.43 |
| Solubility | DMSO: 5 mg/mL (14.27 mM) | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.8536 mL | 14.2682 mL | 28.5364 mL |
| 5 mM | 570.7 μL | 2.8536 mL | 5.7073 mL |
| 10 mM | 285.4 μL | 1.4268 mL | 2.8536 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 37 reference(s) in Google Scholar.)