DL-Ethionine |
Catalog No.GD11710
One-Click Copy Product Info
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DL-Ethionine, an antagonist of Methionine, is toxic and a carcinogen.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 67-21-0
Sample solution is provided at 25 µL, 10mM.
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DL-Ethionine, an antagonist of Methionine, is toxic and a carcinogen [1]. DL-Ethionine could increase the cyclic AMP content and stimulate the malignant transformation of cells [2]. DL-Ethionine has been widely used to induce hepatocarcinogenesis in rats[3].
In vivo, Feeding a low-choline diet containing 0.5% DL-ethionine resulted in the development of acute hemorrhagic pancreatitis with peritoneal fat necrosis in female mice within five days[4]. DL-Ethionine treatment via intraperitoneal injections for 10 days of DL-Ethionine (0.7mg/g) caused degenerative changes in rat pancreatic acinar tissue, including a widening of the endoplasmic reticulum, a reduction in the number of membrane-bound ribosomes, and the appearance of fine and bulky vacuoles containing nongranular, disordered membranes[5]. C3H female mice given 150mg/kg DL-Ethionine by intubation three times a week for 30 weeks resulted in a significant increase in the incidence of liver tumors[6].
References:
[1] Wang J, Yao H, Lu T, et al. Spectroscopic, kinetic, and theoretical analyses of oxidation of dl-ethionine by Pt (IV) anticancer model compounds[J]. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 2019, 223: 117328.
[2] DeRubertis F R, Craven P A. Sequential alterations in the hepatic content and metabolism of cyclic AMP and cyclic GMP induced by DL-ethionine: Evidence for malignant transformation of liver with a sustained increase in cyclic AMP[J]. Metabolism, 1976, 25(12): 1611-1625.
[3] Kamamoto Y, Makiura S, Sugihara S, et al. The inhibitory effect of copper on DL-ethionine carcinogenesis in rats[J]. Cancer research, 1973, 33(5): 1129-1135.
[4] Lombardi B, Estes L W, Longnecker D S. Acute hemorrhagic pancreatitis (massive necrosis) with fat necrosis induced in mice by DL-ethionine fed with a choline-deficient diet[J]. The American journal of pathology, 1975, 79(3): 465.
[5] Herman L, Fitzgerald P J. The degenerative changes in pancreatic acinar cells caused by DL-ethionine[J]. The Journal of Cell Biology, 1962, 12(2): 277-296.
[6] Hoover K L, Hyde C L, Wenk M L, et al. Ethionine carcinogenesis in CD-1, BALB/c and C3H mice[J]. Carcinogenesis, 1986, 7(7): 1143-1148.
| Animal experiment [1]: | |
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Animal models |
Male albino rats |
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Preparation Method |
One hundred and fifty male albino rats, Wistar strain, weighing 160 to 180g, were given water ad libitum and kept in individual cages in an air-conditioned room at 68°F. One hundred and twenty-five of the rats were placed on a protein-free diet. Seventy-five of the rats on the protein-free diet received daily intraperitoneal injections for 10 days of DL-Ethionine (0.7mg/g) dissolved in aqueous 0.9 sodium chloride (ethionine group). Fifty mice were kept on the protein-free diet. After day 10, the mice of both the DL-Ethionine and protein-free groups were maintained on the protein-free diet through day 28. The status of pancreatic acinar tissues in rats injected with ethionine was analyzed. |
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Dosage form |
0.7mg/g/day for 10 days; i.p. |
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Applications |
DL-Ethionine treatment caused degenerative changes in rat pancreatic acinar tissue, including a widening of the endoplasmic reticulum, a reduction in the number of membrane-bound ribosomes, and the appearance of fine and bulky vacuoles containing nongranular, disordered membranes. |
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References: |
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| Cas No. | 67-21-0 | SDF | |
| Formula | C6H13NO2S | M.Wt | 163.238 |
| Solubility | Storage | -20°C, protect from light, away from moisture | |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 6.126 mL | 30.6301 mL | 61.2602 mL |
| 5 mM | 1.2252 mL | 6.126 mL | 12.252 mL |
| 10 mM | 612.6 μL | 3.063 mL | 6.126 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)