Sulfo-SANPAH |
Catalog No.GD21201
One-Click Copy Product Info
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Sulfo-SANPAH is a water-soluble, heterobifunctional crosslinker; Sulfo-SANPAH contains a primary amine-reactive NHS ester and a photoactivatable nitrophenyl azide group.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 102568-43-4
Sample solution is provided at 25 µL, 10mM.
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Sulfo-SANPAH is a primary amine-nitrophenyl azide crosslinker[1]. Sulfo-SANPAH is a heterobifunctional crosslinker with one end containing a phenyl azide group and the other end featuring an amine-reactive N-hydroxysuccinimide ester, with an arm length of 18.2 A[2]. Sulfo-SANPAH is photosensitive and can form covalent bonds between proteins or other biomolecules upon activation with ultraviolet light[3]. Sulfo-SANPAH can be used for crosslinking cell surface proteins, and it is highly soluble in water. For instance, Sulfo-SANPAH can be employed to facilitate the covalent bonding of cell adhesion proteins to the surface of culture mediums, thereby studying cell migration and interactions with the extracellular matrix[4].
References:
[1] Hong Y, Krsko P, Libera M. Protein surface patterning using nanoscale PEG hydrogels[J]. Langmuir, 2004, 20(25): 11123-11126.
[2] Uckun F M, Evans W E, Forsyth C J, et al. Biotherapy of B-cell precursor leukemia by targeting genistein to CD19-associated tyrosine kinases[J]. Science, 1995, 267(5199): 886-891.
[3] Kumai J, Sasagawa S, Horie M, et al. A novel method for polyacrylamide gel preparation using N-hydroxysuccinimide-acrylamide ester to study cell-extracellular matrix mechanical interactions[J]. Frontiers in Materials, 2021, 8: 637278.
[4]Millar-Haskell C S, Gleghorn J P. A Large-format Polyacrylamide Gel with Controllable Matrix Mechanics for Mammalian Cell Culture and Conditioned Media Production[J]. Bio-protocol, 2023, 13(17).
This plan only provides a guide, please modify it to meet your specific needs.
1. Solution Preparation
(1) Working solution: Weigh the solid and prepare the required working concentration with Milli-Q water or other suitable buffer.
Note: Store solids at -20°C or -80°C protected from light. Please adjust the optimal working concentration according to the actual situation or refer to the literature to set the gradient concentration yourself to explore. The working fluid must be prepared and used immediately.
2.Using Sulfo-SANPAH for extracellular matrix protein-polyacrylamide conjugation[1] (from the literature, for reference only)
(1)In HBS buffer, dilute the selected extracellular matrix protein (e.g., collagen) to a final concentration of 100μg/mL, preparing the necessary volume of working solution.
(2) Remove all buffer from the polyacrylamide culture dish.
(3) Dilute Sulfo-SANPAH in Milli-Q water to 0.5mg/mL. Immediately use a pipette to evenly spread about 5mL of the solution over the surface of the polyacrylamide gel. Since Sulfo-SANPAH hydrolyzes rapidly in water, this step must be completed quickly.
(4) Open the culture dish lid and expose the polyacrylamide dish to 365nm ultraviolet light for 10min to activate the Sulfo-SANPAH. After treatment, the polyacrylamide should appear a deep orange color.
(5) Thoroughly wash the polyacrylamide gel three times with HBS buffer to remove any unreacted Sulfo-SANPAH.
(6) Directly add the protein solution to the surface of the polyacrylamide, ensuring the liquid completely covers the gel. Transfer the dish to a 37°C incubator, incubate overnight or for at least 2-3 hours.
(7) After incubating with the protein solution, wash once with HBS and replace with fresh HBS.
(8) Incubate the treated polyacrylamide gel in HBS at 37°C overnight or for at least 2-3 hours to stabilize the protein conjugation.
(9) The treated culture dish can be stored at 4°C, sealed with parafilm or plastic wrap for several weeks.
Note:The choice of extracellular matrix protein (fibronectin, collagen, laminin, etc.) should be based on the target cell line and tested for compatibility. Always ensure aseptic techniques are used throughout the process to prevent contamination.
References:
[1] Millar-Haskell C S, Gleghorn J P. A Large-format Polyacrylamide Gel with Controllable Matrix Mechanics for Mammalian Cell Culture and Conditioned Media Production[J]. Bio-protocol, 2023, 13(17).
| Cas No. | 102568-43-4 | SDF | |
| Formula | C16H18N6O9S | M.Wt | 470.41 |
| Solubility | 60mg/mL in DMSO (Need ultrasonic) | Storage | -20℃ |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.1258 mL | 10.629 mL | 21.2581 mL |
| 5 mM | 425.2 μL | 2.1258 mL | 4.2516 mL |
| 10 mM | 212.6 μL | 1.0629 mL | 2.1258 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)