IPPD-Q (Synonyms: N-Isopropyl-N'-phenyl-p-phenylenediamine-quinone) |
Catalog No.GC91672
One-Click Copy Product Info
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IPPD-Q is the oxidation products of N,N'-Substituted p-phenylenediamines (PPD), with oxidative potential values of 0.93μM min–1μM–1for dithiothreitol (DTT) consumption rate.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 68054-73-9
Sample solution is provided at 25 µL, 10mM.
IPPD-Q is the oxidation products of N,N'-Substituted p-phenylenediamines (PPD), with oxidative potential values of 0.93μM min–1μM–1for dithiothreitol (DTT) consumption rate[1-2]. IPPD-Q has been used in air pollution research and pollutant diagnosis research[3].
In vitro, IPPD-Q exhibits EC50 values of 2.97mg/L (30min) for Aquatic bacterium Vibrio fischeri[4]. IPPD-Q treatment (2.97mg/L) for 30min resulted in a significant decrease in metabolic activity and esterase activity and an increase in the degree of membranocyte damage and oxidative stress in Vibrio fischeri[4]. Treatment of hCMEC-D3 cells with 1.00μM IPPD-Q for 24 hours induced cell apoptosis, endothelial-mesenchymal transition, upregulated Plasminogen activator inhibitor-1 (PAI-1) expression, and triggered blood-brain barrier dysfunction[5].
References:
[1] Wang W, Cao G, Zhang J, et al. p-Phenylenediamine-derived quinones as new contributors to the oxidative potential of fine particulate matter[J]. Environmental Science & Technology Letters, 2022, 9(9): 712-717.
[2] Wang W, Cao G, Zhang J, et al. Beyond substituted p-Phenylenediamine antioxidants: Prevalence of their quinone derivatives in PM2. 5[J]. Environmental Science & Technology, 2022, 56(15): 10629-10637.
[3] Mao T, Liu W, Deng J, et al. p-Phenylenediamines and p-phenylenediamine quinone derivatives in rubber consumer products and typical urban dust: Sources, transformation profiles, and health risks[J]. Environment International, 2024, 192: 109042.
[4] Wang W, Chen Y, Fang J, et al. Toxicity of substituted p-phenylenediamine antioxidants and their derived novel quinones on aquatic bacterium: Acute effects and mechanistic insights[J]. Journal of Hazardous Materials, 2024, 469: 133900.
[5] Han M, Xia K, Xue Y, et al. Emergence of More Potent PPD-Qs beyond 6PPD-Q in Human Blood and Cerebrospinal Fluid[J]. Environmental Science & Technology Letters, 2025.
| Cell experiment [1]: | |
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Cell lines |
hCMEC-D3 cell |
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Preparation Method |
hCMEC-D3 cells were grown in endothelial cell medium (ECM) which included 5% fetal bovine serum (FBS) and 1% endothelial cell growth supplement along with 1% penicillin/streptomycin solution. Under 37 °C conditions with 5% CO2 humidified atmosphere the cell culture medium was changed every two days until cells reached 90% confluency. For 24 hours cells underwent treatment with different concentrations of IPPD-Q including 0, 0.01, 0.10, and 1.00μM. Collect cells from various exposure groups for protein expression analysis using Western blotting assays. Assess whether atorvastatin at a concentration of 0.30μM could modify the cellular effects of IPPD-Q when cells received both treatments simultaneously. The vehicle control that consisted of 0.1% DMSO (v/v) showed negligible effects after its evaluation process and thus became the designated exposure group 0. |
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Reaction Conditions |
0, 0.01, 0.10, and 1.00μM; 24h |
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Applications |
IPPD-Q can increase the level of TNF-α, induce cell apoptosis, up-regulate PAI-1 and lead to endothelial-to-mesenchymal transition of hCMEC-D3 cells, resulting in endothelial cell dysfunction. |
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References: |
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| Cas No. | 68054-73-9 | SDF | |
| Synonyms | N-Isopropyl-N'-phenyl-p-phenylenediamine-quinone | ||
| Formula | C15H16N2O2 | M.Wt | 256.3 |
| Solubility | Acetonitrile: Slightly soluble: 0.1-1 mg/ml,DMSO: Sparingly soluble: 1- 10 mg/ml | Storage | -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 3.9017 mL | 19.5084 mL | 39.0168 mL |
| 5 mM | 780.3 μL | 3.9017 mL | 7.8034 mL |
| 10 mM | 390.2 μL | 1.9508 mL | 3.9017 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)