IF 12
PMID: 41192036 ↗IPPD-Q
Catalog No.: GC91672Purity:>98.00%Synonyms: N-Isopropyl-N'-phenyl-p-phenylenediamine-quinone
IPPD-Q is the oxidation products of N,N'-Substituted p-phenylenediamines (PPD), with oxidative potential values of 0.93μM min–1μM–1for dithiothreitol (DTT) consumption rate.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 68054-73-9
1 mgIn Stock
$73.00
1
5 mgIn Stock
$328.00
1
10 mgIn Stock
$582.00
1
25 mgIn Stock
$1,274.00
1
| Pack Size | Price | Availability | Quantity | Action |
|---|---|---|---|---|
| 1 mg | $73.00 | In Stock | 1 | |
| 5 mg | $328.00 | In Stock | 1 | |
| 10 mg | $582.00 | In Stock | 1 | |
| 25 mg | $1,274.00 | In Stock | 1 |
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Citations
1 publicationsPublications from researchers using this productDescription
IPPD-Q is the oxidation products of N,N'-Substituted p-phenylenediamines (PPD), with oxidative potential values of 0.93μM min–1μM–1for dithiothreitol (DTT) consumption rate[1-2]. IPPD-Q has been used in air pollution research and pollutant diagnosis research[3].
In vitro, IPPD-Q exhibits EC50 values of 2.97mg/L (30min) for Aquatic bacterium Vibrio fischeri[4]. IPPD-Q treatment (2.97mg/L) for 30min resulted in a significant decrease in metabolic activity and esterase activity and an increase in the degree of membranocyte damage and oxidative stress in Vibrio fischeri[4]. Treatment of hCMEC-D3 cells with 1.00μM IPPD-Q for 24 hours induced cell apoptosis, endothelial-mesenchymal transition, upregulated Plasminogen activator inhibitor-1 (PAI-1) expression, and triggered blood-brain barrier dysfunction[5].
References:
[1] Wang W, Cao G, Zhang J, et al. p-Phenylenediamine-derived quinones as new contributors to the oxidative potential of fine particulate matter[J]. Environmental Science & Technology Letters, 2022, 9(9): 712-717.
[2] Wang W, Cao G, Zhang J, et al. Beyond substituted p-Phenylenediamine antioxidants: Prevalence of their quinone derivatives in PM2. 5[J]. Environmental Science & Technology, 2022, 56(15): 10629-10637.
[3] Mao T, Liu W, Deng J, et al. p-Phenylenediamines and p-phenylenediamine quinone derivatives in rubber consumer products and typical urban dust: Sources, transformation profiles, and health risks[J]. Environment International, 2024, 192: 109042.
[4] Wang W, Chen Y, Fang J, et al. Toxicity of substituted p-phenylenediamine antioxidants and their derived novel quinones on aquatic bacterium: Acute effects and mechanistic insights[J]. Journal of Hazardous Materials, 2024, 469: 133900.
[5] Han M, Xia K, Xue Y, et al. Emergence of More Potent PPD-Qs beyond 6PPD-Q in Human Blood and Cerebrospinal Fluid[J]. Environmental Science & Technology Letters, 2025.
Protocol
| Cell experiment [1]: | |
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Cell lines |
hCMEC-D3 cell |
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Preparation Method |
hCMEC-D3 cells were grown in endothelial cell medium (ECM) which included 5% fetal bovine serum (FBS) and 1% endothelial cell growth supplement along with 1% penicillin/streptomycin solution. Under 37 °C conditions with 5% CO2 humidified atmosphere the cell culture medium was changed every two days until cells reached 90% confluency. For 24 hours cells underwent treatment with different concentrations of IPPD-Q including 0, 0.01, 0.10, and 1.00μM. Collect cells from various exposure groups for protein expression analysis using Western blotting assays. Assess whether atorvastatin at a concentration of 0.30μM could modify the cellular effects of IPPD-Q when cells received both treatments simultaneously. The vehicle control that consisted of 0.1% DMSO (v/v) showed negligible effects after its evaluation process and thus became the designated exposure group 0. |
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Reaction Conditions |
0, 0.01, 0.10, and 1.00μM; 24h |
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Applications |
IPPD-Q can increase the level of TNF-α, induce cell apoptosis, up-regulate PAI-1 and lead to endothelial-to-mesenchymal transition of hCMEC-D3 cells, resulting in endothelial cell dysfunction. |
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References: |
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Product Documents
Purity:>98.00%Appearance:A solid
Chemical Properties
CAS No.
68054-73-9
Synonyms
N-Isopropyl-N'-phenyl-p-phenylenediamine-quinone
Molecular Formula
C15H16N2O2
Molecular Weight
256.3
Solubility
Acetonitrile: Slightly soluble: 0.1-1 mg/ml,DMSO: Sparingly soluble: 1- 10 mg/ml
Storage
-20°C
General tips
Select a suitable solvent according to the product solubility data. Once prepared, aliquot the stock solution to avoid repeated freeze-thaw cycles.
Use stock solutions within 6 months when stored at -80°C or within 1 month when stored at -20°C.
To improve solubility, warm the tube to 37°C and sonicate it for an appropriate period.
Use stock solutions within 6 months when stored at -80°C or within 1 month when stored at -20°C.
To improve solubility, warm the tube to 37°C and sonicate it for an appropriate period.
Shipping Condition
Evaluation samples are shipped with blue ice. Other available sizes are shipped at room temperature or with blue ice upon request.
CalculatorsMolarity / Dilution / Molecular Weight / Unit Conversion / In Vivo Formulation / Solubility
g/mol
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