C9-200 is an ionizable cationic lipid that has been used in the formation of lipid nanoparticles (LNPs).1 LNPs containing C9-200 and encapsulating mRNA encoding erythropoietin (EPO) increase serum EPO levels in mice. LNPs containing C9-200 and encapsulating mRNA encoding the Cas9 nuclease and small-guide RNA (sgRNA) targeting transthyretin (TTR) induce 3-fold higher hepatic insertion and deletion (indel) formation than LNPs containing C12-200 and encapsulating mRNA encoding Cas9 in mice.
References:
[1]. Mrksich, K., Padilla, M.S., Joseph, R.A., et al.Influence of ionizable lipid tail length on lipid nanoparticle delivery of mRNA of varying lengthJ. Biomed. Mater. Res. A112(9)1494-1505(2024).