DIMBOA |
カタログ番号GC60138
One-Click Copy Product Info
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Dimboa（2,4-ジヒドロキシ-7-メトキシ-2H-1,4-ベンゾキサジン-3（4H）-ONE）はベンゾキサジノイド（BX）であり、草の二次代謝産物である。
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 15893-52-4
Sample solution is provided at 25 µL, 10mM.
Dimboa（2,4-ジヒドロキシ-7-メトキシ-2H-1,4-ベンゾキサジン-3（4H）-ONE）はベンゾキサジノイド（BX）であり、草の二次代謝産物である[1]。Dimboaはトウモロコシ性植物の化学防御システムの一部である抗生物質とアレロケミカルとして機能する[1,2]。Dimboaは黄色ブドウ球菌、大腸菌、ワイン酵母に類を見ない研究された細菌や真菌の多くの株に対して成長に阻害特性がある[2]。一度リリースすると、BXSは水性環境で比較的迅速に劣化し、半減期は24時間未満である[1]。
Dimboaは弱い細菌変異体であり、なお、人の肝細胞（HEPG2細胞）で異数性を引き起こす[3]。DimboaはHEPG2細胞系における遺伝毒性の効果を示す[4]。DimboaはRalstonia solanacearumに対する抗菌活性が示し、最低阻害濃度（MIC）は50、100と200 mg/lである。 Dimboaは細菌の成長にも顕著に影響し、バイオフィルムの形成を減少させ、２４時間内に群れの運動性を阻害する[5]。
References:
[1]. Neal A L, Ahmad S, Gordon-Weeks R, et al. Benzoxazinoids in root exudates of maize attract Pseudomonas putida to the rhizosphere[J]. PloS one, 2012, 7(4): e35498.
[2]. Gleńsk M, Gajda B, Franiczek R, et al. In vitro evaluation of the antioxidant and antimicrobial activity of DIMBOA [2, 4-dihydroxy-7-methoxy-2 H-1, 4-benzoxazin-3 (4 H)-one][J]. Natural product research, 2016, 30(11): 1305-1308.
[3]. Buchmann C A, Nersesyan A, Kopp B, et al. Dihydroxy-7-methoxy-1, 4-benzoxazin-3-one (DIMBOA) and 2, 4-dihydroxy-1, 4-benzoxazin-3-one (DIBOA), two naturally occurring benzoxazinones contained in sprouts of Gramineae are potent aneugens in human-derived liver cells (HepG2)[J]. Cancer letters, 2007, 246(1-2): 290-299.
[4]. Buchmann C, Nersesyan A, Kopp B, et al. DIMBOA and DIBOA, two naturally occuring benzoxazinoides, cause aneuploidy in a human-derived liver cell line (HepG2)[C]//BMC Pharmacology. BioMed Central, 2007, 7(2): 1-1.
[5]. Guo B, Zhang Y, Li S, et al. Extract from maize (Zea mays L.): antibacterial activity of DIMBOA and its derivatives against Ralstonia solanacearum[J]. Molecules, 2016, 21(10): 1397.
| 動物実験 [1]: | |
動物モデル | Pseudomonas putida KT2440 |
準備方法 | P. putida kt2440細胞は5 µgML-1の最終濃度にDIMBOAを添加する前に100 mL M9培地で中期拡張期間まで成長する。1時間の暴露後、細胞を4°Cで遠心分離することにより採取する。 |
投与形態 | 5 µg ML-1，1H |
アプリケーション | 他の土壌細菌と比べると、P. putida kt2440はDimboaに対して比較的耐性が弱い。その後のすべての実験では、Dimboaは5 µg ML-1（0.023 mM）の濃度で使う。 |
References: | |
| Cas No. | 15893-52-4 | SDF | |
| Canonical SMILES | O=C1C(O)OC2=CC(OC)=CC=C2N1O | ||
| Formula | C9H9NO5 | M.Wt | 211.17 |
| 溶解度 | DMSO: 125 mg/mL (591.94 mM) | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 4.7355 mL | 23.6776 mL | 47.3552 mL |
| 5 mM | 947.1 μL | 4.7355 mL | 9.471 mL |
| 10 mM | 473.6 μL | 2.3678 mL | 4.7355 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 13 reference(s) in Google Scholar.)