GR119566X is a 5-HT 3 receptor ligand with a pK i value of 8.80 against prufied 5-HT 3 receptor. GR119566X can be used for 5-HT 3 receptor purification [1].
In Vitro, GR119566X (12.5 mg; 16 h) is successfully coupled to Affi-Gel 15 to form an affinity resin that specifically retains solubilized 5-HT3 receptors from porcine cerebral cortex, enabling receptor purification[1]. GR119566X (60 min at 37°C) binds to membrane-bound, solubilized, and affinity-purified 5-HT3 receptors from porcine cerebral cortex with consistent high affinity, as shown by pKi values ranging from 8.21 to 8.80[1].
References:
[1]. Fletcher S, et al. Purification of 5-hydroxytryptamine3 receptors from porcine brain. Br J Pharmacol. 1997 Oct;122(4):655-62.
In Vitro, GR119566X (12.5 mg; 16 h) is successfully coupled to Affi-Gel 15 to form an affinity resin that specifically retains solubilized 5-HT3 receptors from porcine cerebral cortex, enabling receptor purification[1]. GR119566X (60 min at 37°C) binds to membrane-bound, solubilized, and affinity-purified 5-HT3 receptors from porcine cerebral cortex with consistent high affinity, as shown by pKi values ranging from 8.21 to 8.80[1].
References:
[1]. Fletcher S, et al. Purification of 5-hydroxytryptamine3 receptors from porcine brain. Br J Pharmacol. 1997 Oct;122(4):655-62.