Influenza Hemagglutinin (HA) Peptide (Synonyms: H-Tyr-Pro-Tyr-Asp-Val-Pro-Asp-Tyr-Ala-OH) |
カタログ番号GP10152 |
ペプチド
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 92000-76-5
Sample solution is provided at 25 µL, 10mM.
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
HAタグ、プラスミドDNAを含む複合体、トランスフェリン-ポリリシン共役体、およびインフルエンザウイルス血凝固素サブユニットHA-2のN末端配列から派生したポリリシン共役ペプチドは、ルシフェラーゼまたはβ-ガラクトシダーゼマーカー遺伝子をK562細胞、HeLa細胞、およびBNL CL.2肝細胞に転送するために使用されています。これらのインフルエンザペプチド共役体がDNA複合体中に存在することで、脂質体漏出アッセイで膜破壊活性を示し、トランスフェリン-ポリリシン媒介遺伝子導入の大幅な増強効果が得られます。
インフルエンザウイルスでは、血凝固素(HA)タンパク質がウイルスの細胞表面への結合とその後のウイルスと細胞膜の融合を仲介します。 HAは受容体結合サブユニットであるHA1と融合性サブユニットであるHA2から構成されています。ネイティブのHA1yHA2複合体は、ネイティブウイルス表面に存在するときには融合が非活性です。インフルエンザウイルスでは、細胞内エンドソームの酸性環境にさらされたときに、血凝固素(HA)タンパク質の立体構造がネイティブ(非融合性)から融合活性(融合性)へ切り替わることで、細胞膜の融合が調節されます。 HAのネイティブ構造は不安定な状態で閉じ込められており、 融解能を持つ形状へ変化するためにはこの不安定な状態を解除しなければなりません。
References:
1. E. WAGNER, C. PLANK et al, Influenza virus hemagglutinin HA-2 N-terminal fusogenic peptides augment gene transfer by transferrin-polylysine-DNA complexes: Toward a synthetic virus-like gene-transfer vehicle. Proc. Nati. Acad. Sci. USA Vol. 89, pp. 7934-7938, September 1992
2. C. M. CARR, C. CHAUDHRY, P. S. KIM et al. Influenza hemagglutinin is spring-loaded by a metastable native conformation. Proc. Natl. Acad. Sci. USA Vol. 94, pp. 14306–14313
Cas No. | 92000-76-5 | SDF | |
同義語 | H-Tyr-Pro-Tyr-Asp-Val-Pro-Asp-Tyr-Ala-OH | ||
Chemical Name | Influenza Hemagglutinin (HA) Peptide | ||
Canonical SMILES | CC(C)C(C(=O)N1CCCC1C(=O)NC(CC(=O)O)C(=O)NC(CC2=CC=C(C=C2)O)C(=O)NC(C)C(=O)O)NC(=O)C(CC(=O)O)NC(=O)C(CC3=CC=C(C=C3)O)NC(=O)C4CCCN4C(=O)C(CC5=CC=C(C=C5)O)N | ||
Formula | C53H67N9O17 | M.Wt | 1102.15 |
溶解度 | ≥55.1075mg/mL in DMSO, ≥100.4 mg/mL in EtOH, ≥46.2 mg/mL in Water | Storage | Desiccate at -20°C |
General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. |
Prepare stock solution | |||
1 mg | 5 mg | 10 mg | |
1 mM | 0.9073 mL | 4.5366 mL | 9.0732 mL |
5 mM | 0.1815 mL | 0.9073 mL | 1.8146 mL |
10 mM | 0.0907 mL | 0.4537 mL | 0.9073 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
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Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
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