dADP disodium (2'-Deoxyadenosine 5'-di-phos-phate disodium)

dADP disodium (2'-Deoxyadenosine 5'-di-phos-phate disodium) is a purine nucleotide diphosphate related to ADP and dATP^[1]. dADP disodium can act as a substrate analog and inhibitor in polynucleotide phosphorylase reactions, including inhibition of Adenosine diphosphate(ADP) and Cytidine diphosphate(CDP) polymerization at 0.23mM in bacterial enzyme systems^[1,2]. dADP disodium is used in research on nucleotide metabolism, polymerase and phosphorylase specificity, purinergic cell responses, and insect chemosensory feeding assays^[1-5].

In vitro, human platelet-rich plasma exposed to dADP disodium (7μg/mL; single addition) showed platelet-aggregating activity. On a weight basis, its activity was approximately 0.3% of that of ADP, supporting its use as a deoxy-nucleotide probe in platelet-response research^[3].

In vivo, adult female Culex pipiens and Culiseta inornata were tested in artificial feeding assays with dADP disodium (1mM; single artificial-diet exposure). dADP disodium produced a weaker phagostimulatory response than ADP^[4].

References:
[1] Lucas-Lenard JM, Cohen SS. The inhibition of polynucleotide phosphorylase by certain substrate analogues. Biochim Biophys Acta. 1966;123(3):471-477. doi:10.1016/0005-2787(66)90209-0.
[2] Chou JY, Singer MF. Deoxyadenosine diphosphate as a substrate and inhibitor of polynucleotide phosphorylase of Micrococcus luteus. II. Inhibition of the initiation of adenosine diphosphate polymerization by deoxyadenosine diphosphate. J Biol Chem. 1971;246(24):7497-7504.
[3] Gaarder A, Laland S. Hypothesis for the aggregation of platelets by nucleotides. Nature. 1964;202:909-910. doi:10.1038/202909a0.
[4] Galun R, Friend WG, Nudelman S. Purinergic reception by culicine mosquitoes. J Comp Physiol A. 1988;163:665-670. doi:10.1007/BF00603850.
[5] Yazawa S, Tsuchiya H, Hori H, Makino R. Functional characterization of two nucleotide-binding sites in soluble guanylate cyclase. J Biol Chem. 2006;281(31):21763-21770. doi:10.1074/jbc.M602790200.