DC-Chol (hydrochloride) (Synonyms: Cholesteryl 3β-N-(dimethylaminoethyl)carbamate) |
| Catalog No.GC43385 |
DC-Chol (hydrochloride)은 Aβ40 섬유체 형성을 억제할 수 있으며, DC-Chol(hydrochloric acid)은 산화된 hCT의 엠파이로이드 형성을 억제할 수 있다.
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Cas No.: 166023-21-8
Sample solution is provided at 25 µL, 10mM.
DC-Chol (hydrochloride)은 Aβ40 섬유체 형성을 억제할 수 있으며, DC-Chol(hydrochloric acid)은 산화된 hCT의 엠파이로이드 형성을 억제할 수 있다.
DC-Chol(hydrochloric acid)에 대해서, Aβ40(30 µM)가 낮은 농도의 DC-Chol(hydrochloride)(50 ng/mL)와 공존시킨 이미지에서 Aβ 섬유체가 관찰되었다. 하지만 500 µg/mL DC-Chol(hydrochloride)이 존재하는 상황에서 이미지에 섬유 구조가 발견되지 않았다. DC-Chol/DOPE 양이온 립소섬은 강한 엔트로피 운동에 의한 표면 전기적 상호작용을 통해 CT-DNA를 적절하게 압축하고 밀집시킨다.Calf-thymus DNA(CT-DNA)와 양이온 지질 3β-[N-(N',N'-dimethylaminoethane)-carbamoyl]콜레스테롤 염산염(DC-Chol (hydrochloride))과 DOPE로 구성된 혼합 양이온 립소섬으로 구성된 립플렉스가 분석되었다. DC-Chol/DOPE 립소섬은 평균 유동역학 지름이 약 (120±10) 톤으로, 강한 엔트로피 운동에 의한 전기 상호작용을 통해 양이온 표면에서 DNA 단편을 압축하고 밀집시킨다. 50 mol% DODAC 또는 DC-Chol(hydrochloric acid), 그리고 20 mol% DSPE-PEG(2000)으로 준비한 공식은 순환 반감기를 6.5에서 12.5시간 사이로 나타냈다. DSPE-PEG(2000)으로 준비한 DC-Chol(hydrochloric acid) 공식은 PEG이 없는 대조군보다 피하 HT29 종양에서 세배 더 높은 쌓임을 보였다. DC-Chol(hydrochloric acid)은 거대세포내성 및 그레아틴 중재 내성 작용을 통해 세포 내로 흡수된다.
References:
[1]. Elbassal EA, Liu H, et,al. Effects of Charged Cholesterol Derivatives on Aβ40 Amyloid Formation. J Phys Chem B. 2016 Jan 14;120(1):59-68. doi: 10.1021/acs.jpcb.5b09557. Epub 2015 Dec 23. PMID: 26652010; PMCID: PMC4959543.
[2]. Lantz R, Busbee B, et,al. Effects of disulfide bond and cholesterol derivatives on human calcitonin amyloid formation. Biopolymers. 2020 May;111(5):e23343. doi: 10.1002/bip.23343. Epub 2019 Dec 5. PMID: 31804717; PMCID: PMC9254112.
[3]. RodrÍguez-Pulido A, Ortega F, et,al. A physicochemical characterization of the interaction between DC-Chol/DOPE cationic liposomes and DNA. J Phys Chem B. 2008 Oct 2;112(39):12555-65. doi: 10.1021/jp804066t. Epub 2008 Aug 27. PMID: 18729499.
[4]. MuÑoz-Ubeda M, RodrÍguez-Pulido A, et,al. Effect of lipid composition on the structure and theoretical phase diagrams of DC-Chol/DOPE-DNA lipoplexes. Biomacromolecules. 2010 Dec 13;11(12):3332-40. doi: 10.1021/bm1008124. Epub 2010 Nov 8. PMID: 21058732.
[5]. Rapaka H, Manturthi S, et,al. Effect of Methylation of the Hydrophilic Domain of Tocopheryl Ammonium-Based Lipids on their Nucleic Acid Delivery Properties. ACS Omega. 2022 Apr 29;7(18):15396-15403. doi: 10.1021/acsomega.1c06889. PMID: 35571792; PMCID: PMC9096827.
[6]. Ho EA, Ramsay E, et,al. Characterization of cationic liposome formulations designed to exhibit extended plasma residence times and tumor vasculature targeting properties. J Pharm Sci. 2010 Jun;99(6):2839-53. doi: 10.1002/jps.22043. PMID: 20091826.
| 원자력 현미경 (AFM)[1]: | |
|
베양기 |
Aβ40 |
|
제조 방법 |
pH 7.4의 인산 버퍼에서 Aβ40 (30 μM)이 Speci-Mix 분사 혼합기에서 흡수되었습니다. 50 ng/mL 콜레스테롤-SO4, 500 μg/mL 콜레스테롤-SO4, 50 ng/mL DC-Chol (hydrochloride), 그리고 500 μg/mL DC-Chol이 존재하는 조건에서 Aβ40. |
|
반응 조건 |
50/500 ng/mL DC-Chol (hydrochloride)이 Aβ40과 함께 17시간 동안 흡수되었습니다. |
|
응용 분야 |
DC-Chol (hydrochloride)에 대해서, Aβ40 (30 μM)이 낮은 농도의 DC-Chol (50 ng/mL)와 함께 흡수되는 이미지에서 Aβ 섬유구조가 관찰되었습니다. 하지만 500 μg/mL DC-cholesterol이 존재하는 경우, 이미지에서 섬유 구조가 발견되지 않았습니다. |
|
References: [1]. Elbassal EA, Liu H, et,al. Effects of Charged Cholesterol Derivatives on Aβ40 Amyloid Formation. J Phys Chem B. 2016 Jan 14;120(1):59-68. doi: 10.1021/acs.jpcb.5b09557. Epub 2015 Dec 23. PMID: 26652010; PMCID: PMC4959543. |
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| Cas No. | 166023-21-8 | SDF | |
| Synonyms | Cholesteryl 3β-N-(dimethylaminoethyl)carbamate | ||
| Chemical Name | (3β)-cholest-5-en-3-ol 3-[N-[2-(dimethylamino)ethyl]carbamate], monohydrochloride | ||
| Canonical SMILES | CC(C)CCC[C@@H](C)[C@@]1([H])CC[C@@]2([H])[C@]3([H])CC=C4C[C@@H](OC(NCCN(C)C)=O)CC[C@]4(C)[C@@]3([H])CC[C@@]21C.Cl | ||
| Formula | C32H56N2O2•HCl | M.Wt | 537.3 |
| Solubility | 10mg/mL in methanol, 5mg/mL in DMSO | Storage | Store at -20°C, protect from light |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.8612 mL | 9.3058 mL | 18.6116 mL |
| 5 mM | 372.2 μL | 1.8612 mL | 3.7223 mL |
| 10 mM | 186.1 μL | 930.6 μL | 1.8612 mL |
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >97.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 38 reference(s) in Google Scholar.)
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