DIMBOA |
| Catalog No.GC60138 |
DIMBOA(2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one)은 벤조자신류(BX)의 일종으로서 잔디속에 존재하는 2차 대사물질이다.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 15893-52-4
Sample solution is provided at 25 µL, 10mM.
DIMBOA(2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one)은 벤조자신류(BX)의 일종으로서 잔디속에 존재하는 2차 대사물질이다. DIMBOA는 항생제와 화학적 방어계통의 일부이고 옥수수, 밀, 라면과 같은 밀과목 식물의 화학적 방어계통의 일부이다[1,2]. DIMBOA는 황금콩균, 대장균, 맥주효모와 같은 많은 박테리아와 곰팡의 성장을 억제하는 성질을 가지고 있다[2]. 방출되면 BX는 수용액 환경에서 상대적으로 빠르게 분해되고 반감기가 24시간 미만이다[1].
DIMBOA는 약한 박테리아 변이물질이고 더욱이 인간에서 유래한 간세포(HepG2 세포)에서 비정상적인 세포핵수를 유발한다[3]. DIMBOA는 HepG2 세포계에서 유전독성 효과를 보였다[4].
DIMBOA는 녹각균에 대한 항생활성을 나타내고 최소 억제 농도(MICs)가 50, 100, 200 mg/L이다. DIMBOA는 또한 박테리아 성장에 영향을 미치고 생물막 형성을 줄이고 24시간 내에 군집 이동성을 억제한다[5].
References:
[1]. Neal A L, Ahmad S, Gordon-Weeks R, et al. Benzoxazinoids in root exudates of maize attract Pseudomonas putida to the rhizosphere[J]. PloS one, 2012, 7(4): e35498.
[2]. Gleńsk M, Gajda B, Franiczek R, et al. In vitro evaluation of the antioxidant and antimicrobial activity of DIMBOA [2, 4-dihydroxy-7-methoxy-2 H-1, 4-benzoxazin-3 (4 H)-one][J]. Natural product research, 2016, 30(11): 1305-1308.
[3]. Buchmann C A, Nersesyan A, Kopp B, et al. Dihydroxy-7-methoxy-1, 4-benzoxazin-3-one (DIMBOA) and 2, 4-dihydroxy-1, 4-benzoxazin-3-one (DIBOA), two naturally occurring benzoxazinones contained in sprouts of Gramineae are potent aneugens in human-derived liver cells (HepG2)[J]. Cancer letters, 2007, 246(1-2): 290-299.
[4]. Buchmann C, Nersesyan A, Kopp B, et al. DIMBOA and DIBOA, two naturally occuring benzoxazinoides, cause aneuploidy in a human-derived liver cell line (HepG2)[C]//BMC Pharmacology. BioMed Central, 2007, 7(2): 1-1.
[5]. Guo B, Zhang Y, Li S, et al. Extract from maize (Zea mays L.): antibacterial activity of DIMBOA and its derivatives against Ralstonia solanacearum[J]. Molecules, 2016, 21(10): 1397.
| 세포 실험 [1]: | |
세포 라인 | 녹농균KT2440 |
제조 방법 | DIMBOA를 최종 농도 5 µg/mL로 추가하기 전에 P. putida KT2440 세포는 100 mL M9 매체에서 중간 지수 성장기로 성장했습니다. 1시간의 노출 후 세포는 4°C에서 원심분리하여 수확되었습니다. |
반응 조건 | 5 µg mL-1, 1시간 동안 |
응용 분야 | 다른 토양세균에 비해 P. putida KT2440는 DIMBOA에 상대적으로 견딜 수 있습니다. 모든 후속 실험에서 DIMBOA는 5 µg/mL (0.023 mM)의 농도로 사용되었습니다. |
참고문헌: [1]: Neal A L, Ahmad S, Gordon-Weeks R, et al. Benzoxazinoids in root exudates of maize attract Pseudomonas putida to the rhizosphere[J]. PloS one, 2012, 7(4): e35498. | |
| Cas No. | 15893-52-4 | SDF | |
| Canonical SMILES | O=C1C(O)OC2=CC(OC)=CC=C2N1O | ||
| Formula | C9H9NO5 | M.Wt | 211.17 |
| Solubility | DMSO: 125 mg/mL (591.94 mM) | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 4.7355 mL | 23.6776 mL | 47.3552 mL |
| 5 mM | 947.1 μL | 4.7355 mL | 9.471 mL |
| 10 mM | 473.6 μL | 2.3678 mL | 4.7355 mL |
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Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
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Quality Control & SDS
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- Purity: >99.00%
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