EPO mimetic peptide-1 (EMP-1) acetate, a 20 amino acid peptide. EPO mimetic peptide-1 acetate stimulates cell proliferation, affects cell cycle and induces the production of reticulocytes in vivo [1].
In Vivo, EPO mimetic peptide-1 (1-2 mg; once) acetate induces the production of reticulocytes over the background of endogenous in normal, untreated mice containing endogenous basal levels of EPO mice[1].
In Vitro, EPO mimetic peptide-1 acetate shows inhibition of tracer 125I-labeled EPO binding with an IC50 value of 0.2 μM[1]. EPO mimetic peptide-1 (0-50 mM; 48 h) acetate stimulates proliferation of both TF-1 and B6Sut cells in a dose-dependent manner[1]. EPO mimetic peptide-1 (1 and 10 μM) acetate promots erythroid colony formation from human peripheral blood and murine bone marrow[1]. EPO mimetic peptide-1 (10 μM; 10 min) acetate induces the phosphorylation of proteins[1]. EPO mimetic peptide-1 (1 and 10 μM; 0-18 h) acetate affects cell cycle[1].
References:
[1]. Wrighton NC, et al. Small peptides as potent mimetics of the protein hormone erythropoietin. Science. 1996 Jul 26;273(5274):458-64.
In Vivo, EPO mimetic peptide-1 (1-2 mg; once) acetate induces the production of reticulocytes over the background of endogenous in normal, untreated mice containing endogenous basal levels of EPO mice[1].
In Vitro, EPO mimetic peptide-1 acetate shows inhibition of tracer 125I-labeled EPO binding with an IC50 value of 0.2 μM[1]. EPO mimetic peptide-1 (0-50 mM; 48 h) acetate stimulates proliferation of both TF-1 and B6Sut cells in a dose-dependent manner[1]. EPO mimetic peptide-1 (1 and 10 μM) acetate promots erythroid colony formation from human peripheral blood and murine bone marrow[1]. EPO mimetic peptide-1 (10 μM; 10 min) acetate induces the phosphorylation of proteins[1]. EPO mimetic peptide-1 (1 and 10 μM; 0-18 h) acetate affects cell cycle[1].
References:
[1]. Wrighton NC, et al. Small peptides as potent mimetics of the protein hormone erythropoietin. Science. 1996 Jul 26;273(5274):458-64.