GLPBio

Nuclease P1

Catalog No.: GF05226
COASDSData Sheet

Nuclease P1 is a single-stranded specific endonuclease, it hydrolyzes nucleic acids into 5'-mononucleotides and cleaves the single-stranded region of a double-stranded nucleic acid.

제품은 연구용으로만 사용됩니다. 인체에 사용할 수 없으며 환자에게 판매하지 않습니다.

Nuclease P1
Cas No.: 54576-84-0
250UIn Stock
$401.00
1
Tel: (909) 407-4943 / (909) 689-7124Email: sales@glpbio.com

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제품 설명 (Description)

Nuclease P1 is a single-stranded specific endonuclease, it hydrolyzes nucleic acids into 5'-mononucleotides and cleaves the single-stranded region of a double-stranded nucleic acid. Nuclease P1 is one of the most well-known single stranded specific nucleases in the field of molecular biology, it is widely used in the pharmaceutical and food industries[1]. Nuclease P1 can be obtained by fermentation of Penicillium citrinum: through extraction process, ultrafiltration concentration, drying and purification, etc.

References:
[1]. Mitiushkina NV, et al. Preparation of Duplex Sequencing Libraries for Archival Paraffin-Embedded Tissue Samples Using Single-Strand-Specific Nuclease P1. Int J Mol Sci. 2022 Apr 21;23(9):4586.

실험 프로토콜 (Protocol)

Reaction Conditions

pH Range: Effective pH Range: 4.0-8.0, Optimal pH Range: 4.5-5.5
Temperature Range: Effective Temperature Range: 35°C-85°C, Optimal Temperature Range: 65°C-75°C

Usage Method

Using yeast nucleic acid (RNA) as substrate: substrate concentration 2%-5%, recommended enzyme dosage 2%-4% (based on substrate dry matter), hydrolysis time 7-9 hours under optimal conditions;
Using yeast as substrate: yeast concentration 10%-12%, nuclease dosage 0.3%-0.5% (based on yeast dry matter), hydrolysis time 7-9 hours under optimal conditions.

Note: Due to differences in application areas and experimental parameters, the actual addition method and dosage of this product should be determined experimentally.

제품 문서 (Product Documents)

Appearance:A solid
COASDSData Sheet

화학적 특성 (Chemical Properties)

CAS 번호
54576-84-0
용해도
H2O : 4.17 mg/mL (ultrasonic and warming and heat to 37°C)
보관
Store at 4°C , do not freeze.
일반 주의사항
제품의 용해도 데이터에 따라 적절한 용매를 선택하십시오. 조제 후에는 반복적인 동결·해동을 피할 수 있도록 저장 용액을 소분하십시오.
저장 용액은 -80°C 보관 시 6개월 이내, -20°C 보관 시 1개월 이내에 사용하십시오.
용해도를 높이려면 튜브를 37°C로 데운 뒤 적절한 시간 동안 초음파 처리하십시오.
배송 조건
평가용 샘플은 아이스팩과 함께 배송됩니다. 그 외 용량은 실온 배송되며, 요청 시 아이스팩과 함께 배송할 수 있습니다.

계산 도구 (Calculators)몰 농도 / 희석 / 분자량 / 단위 변환

g/mol

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