HPPH is a second-generation photosensitizer, which acts as a photodynamic therapy (PDT) agent. Fluorescence image of 4T1 cells incubated with 0.49 μg/mL GO-PEG, 1 μM HPPH (free HPPH) or equivalent amount of GO-PEG-HPPH (1 μM HPPH and 0.49 μg/mL GO-PEG) after 24 h. The cellular uptake of GO-PEG-HPPH and HPPH is investigated with 4T1 murine mammary cancer cells. The cells are incubated with GO-PEG-HPPH and free HPPH at equivalent HPPH concentration (1 μM) for 24 h and then observed with a confocal microscope. Cells treated with GO-PEG-HHPH show a stronger fluorescence signal than those treated with free HPPH. The fluorescence of HPPH is rather weak[1].
Tumors are treated with an immune-enhancing PDT regimen followed by a tumor-controlling PDT regimen can lead to enhancement of anti-tumor immunity, while retaining effective control of primary tumor growth. To test this hypothesis, a combination treatment regimen is devised in which Colo26-HA tumor-bearing BALB/c mice are treated with an HPPH-PDT regimen known to lead to enhanced anti-tumor immunity (0.4 μmoles/kg HPPH followed 18 h later by illumination with 665 nm light for a total dose of 48 J/cm2). Following illumination, mice are rested for 9 days; on the ninth day, mice are injected with HPPH. On day 10 following the first treatment, tumors are treated with a tumor control treatment regimen (illumination with 665 nm light for a total dose of 132 J/cm2 given)[2].
References:
[1]. Rong P, et al. Photosensitizer loaded nano-graphene for multimodality imaging-guided tumor photodynamic therapy. Theranostics. 2014 Jan 15;4(3):229-39.
[2]. Shams M, et al. Development of photodynamic therapy regimens that control primary tumor growth and inhibit secondary disease. Cancer Immunol Immunother. 2015 Mar;64(3):287-97.