IFNAlpha Human 2a Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 165 amino acids and having a molecular mass of 19241 Dalton.The difference between IFNA2A and IFNA2B is in the amino acid present at position 23. IFN-alpha 2a has a lysine at that position 23 while IFN-alpha 2b has arginine.The IFNA2Agene was obtained from human leukocytes.The IFNA2A is purified by proprietary chromatographic techniques.
인용 문헌 (Citations)
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제품 문서 (Product Documents)
화학적 특성 (Chemical Properties)
출처
E. coli
외관
Sterile Filtered White lyophilized (freeze-dried) powder.
배송 조건
Shipped at ice.
동의어
Leukocyte IFN; B cell IFN; Type I IFN; IFNA2; IFN-a 2a.
아미노산 서열
CDLPQTHSLGSRRTLMLLAQMRKISLFSCLKDRHDFGFPQEEFGNQFQKAETIPVLHEM IQQIFNLFSTKDSSAAWDETLLDKFYTELYQQLNDLEACVIQGVGVTETPLMKEDSILAV RKYFQRITLYLKEKKYSPCAWEVVRAEIMRSFSLSTNLQESLRSKE.N-terminal methionine has been completely removed enzymatically.
겉보기 분자량
~19.2 kDa, on SDS-PAGE under reducing conditions. Da
안정성
Upon receiving, this product remains stable for 6 months at lower than -70°C. Upon reconstitution, the product should be stable for 1 week at 4°C or for 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.
생물학적 활성
ED50 < 0.1 ng/ml, measured by a cytotoxicity assay using TF-1 Cells, corresponding to a specific activity of > 1.0 × 107U/mg.
제형
Lyophilized after extensive dialysis against PBS.
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