Methyl 2-(7-hydroxy-2-oxo-2H-chromen-4-yl)acetate, a natural coumarins derivative, is exploited for the synthesis of the switchable fluorescent substrates to be used in bacterial enzyme detection [1] [2] [3].
In Vitro, Methyl 2-(7-hydroxy-2-oxo-2H-chromen-4-yl)acetate (compound 2) exhibits the maximum absorption (λabs~322 nm) and emission (λem~387 nm) wavelengths[2].
References:
[1]. Nascimento JSD, et al. Mapping the Biotransformation of Coumarins through Filamentous Fungi. Molecules. 2019 Sep 29;24(19).
[2]. Lizzul-Jurse A, et al. Readily functionalizable phosphonium-tagged fluorescent coumarins for enhanced detection of conjugates by mass spectrometry. Org Biomol Chem. 2016 Aug 10;14(32):7777-91.
[3]. Giovannini G, et al. Coumarin-based, switchable fluorescent substrates for enzymatic bacterial detection. Talanta. 2018 Oct 1;188:448-453.
In Vitro, Methyl 2-(7-hydroxy-2-oxo-2H-chromen-4-yl)acetate (compound 2) exhibits the maximum absorption (λabs~322 nm) and emission (λem~387 nm) wavelengths[2].
References:
[1]. Nascimento JSD, et al. Mapping the Biotransformation of Coumarins through Filamentous Fungi. Molecules. 2019 Sep 29;24(19).
[2]. Lizzul-Jurse A, et al. Readily functionalizable phosphonium-tagged fluorescent coumarins for enhanced detection of conjugates by mass spectrometry. Org Biomol Chem. 2016 Aug 10;14(32):7777-91.
[3]. Giovannini G, et al. Coumarin-based, switchable fluorescent substrates for enzymatic bacterial detection. Talanta. 2018 Oct 1;188:448-453.