Poly(A) polymerase catalyzes the incorporation of ATP into the RNA 3' end in the form of AMP, that is, adding a polyadenosine tail at the RNA 3' end. Poly(A) polymerase increases RNA stability and increases mRNA translation efficiency in eukaryotic cells. Poly(A) polymerase has high tailing efficiency and can add 20-200 A bases to the 3' end of RNA [1] [2].
In Vitro, Table. Package Contents Specification Type Components Volume Quantity 100 U A Poly(A) Polymerase (5U/μL) 100 U 1 100 U B 10 x Poly(A) Polymerase Buffer 1 mL 1 100 U C ATP (10 mM) 0.1 mL 1
References:
[1]. Li W, et al. The novel poly(A) polymerase Star-PAP is a signal-regulated switch at the 3'-end of mRNAs. Adv Biol Regul. 2013 Jan;53(1):64-76.
[2]. Chen LS, et al. Chain termination and inhibition of mammalian poly(A) polymerase by modified ATP analogues. Biochem Pharmacol. 2010 Mar 1;79(5):669-77.
In Vitro, Table. Package Contents Specification Type Components Volume Quantity 100 U A Poly(A) Polymerase (5U/μL) 100 U 1 100 U B 10 x Poly(A) Polymerase Buffer 1 mL 1 100 U C ATP (10 mM) 0.1 mL 1
References:
[1]. Li W, et al. The novel poly(A) polymerase Star-PAP is a signal-regulated switch at the 3'-end of mRNAs. Adv Biol Regul. 2013 Jan;53(1):64-76.
[2]. Chen LS, et al. Chain termination and inhibition of mammalian poly(A) polymerase by modified ATP analogues. Biochem Pharmacol. 2010 Mar 1;79(5):669-77.