Pro-GA (Synonyms: Pro-glutaryl Alanine) |
| Catalog No.GC91919 |
Pro-GA 는 N-펜디옥사이드-L-아미노산의 전약형태이자 감마-글루타메이트환전이효소(GGCT)의 억제제로서 GGCT는 글루타메이트의 대사에 관여하는 효소이다.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 2222067-12-9
Sample solution is provided at 25 µL, 10mM.
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Pro-GA is a prodrug form of N-glutaryl-L-alanine and an inhibitor of γ-glutamylcyclotransferase (GGCT), an enzyme involved in glutathione metabolism.1 It reduces the viability of RT-112 bladder cancer cells, which have a high expression of GGCT (IC50 = 73.9 ?M), and UM-UC-3 and T24 bladder cancer cells (IC50s = 58.5 and 57 ?M, respectively). It enhances growth inhibition induced by mitomycin C in RT-112 cells. Pro-GA (50, 75, and 100 ?M) also inhibits the proliferation of MCF-7 breast cancer cells.2 It increases the production of reactive oxygen species (ROS) and induces senescence in MCF-7 cells, effects that can be blocked by N-acetylcysteine (NAC). Pro-GA (25 mg/kg) reduces tumor weight in an MCF-7 mouse xenograft model.
References:
[1]. Hanada, E., Kageyama, S., Murai, R., et al.Pro-GA, a novel inhibitor of γ-glutamylcyclotransferase, suppresses human bladder cancer cell growthAnticancer Res.39(4)1893-1898(2019).
[2]. Ii, H., Taniguchi, K., Yoshiya, T., et al.The γ-glutamylcyclotransferase inhibitor Pro-GA induces an antiproliferative effect through the generation of mitochondrial reactive oxygen speciesAnticancer Res.42(9)4311-4317(2022).
| Cas No. | 2222067-12-9 | SDF | |
| Synonyms | Pro-glutaryl Alanine | ||
| Chemical Name | 5-[[(1S)-2-[(acetyloxy)methoxy]-1-methyl-2-oxoethyl]amino]-5-oxo-pentanoic acid, methyl ester | ||
| Canonical SMILES | COC(CCCC(N[C@@H](C)C(OCOC(C)=O)=O)=O)=O | ||
| Formula | C12H19NO7 | M.Wt | 289.3 |
| Solubility | Storage | -20°C | |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
||
| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 3.4566 mL | 17.2831 mL | 34.5662 mL |
| 5 mM | 0.6913 mL | 3.4566 mL | 6.9132 mL |
| 10 mM | 0.3457 mL | 1.7283 mL | 3.4566 mL |
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
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