RT-AM |
Catalog No.GC34435
One-Click Copy Product Info
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RT-AM은 프로 약물 리얼 티올입니다.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 2280796-94-1
Sample solution is provided at 25 µL, 10mM.
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RT-AM is a pro-drug real thiol. Real Thiol is a reversible reaction-based fluorescent probe which can quantitatively monitor the real-time glutathione dynamics in living cells.
To enhance the cell permeability of Real Thiol, the carboxylic acid groups is converted to acetoxymethyl (AM) esters, which are readily hydrolysed by esterases to regenerate Real Thiol inside cells. For cells treated with the RT-AM, real-time ratiometric images of the rapid changes of intracellular GSH concentrations in single cells can be generated by dividing the fluorescence intensity values for the 405 nm channel by the 488 nm channel at each corresponding pixel[1].
[1]. Jiang X, et al. Quantitative real-time imaging of glutathione. Nat Commun. 2017 Jul 13;8:16087.
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Cell experiment: |
The HeLa cell line is grown in DMEM media supplemented with 10% FBS and 1% 1003 Pen Strep. Cells are cultured under a controlled atmosphere (37°C, 5% CO2). Glass bottom dishes are used for cell culture due to confocal scanning requirements. Cells are treated with RT-AM (1 μM with 1% DMSO in DMEM) for 10-15 min before imaging. Confocal images are acquired with 405 nm laser/418–495 nm filter and 488 nm laser/499–615 nm filter. All the microscope settings are kept consistent in each[1]. |
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References: [1]. Jiang X, et al. Quantitative real-time imaging of glutathione. Nat Commun. 2017 Jul 13;8:16087. |
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| Cas No. | 2280796-94-1 | SDF | |
| Canonical SMILES | O=C1OC2=CC(N3CCC3)=CC=C2C=C1/C=C(C#N)/C(N(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)=O | ||
| Formula | C26H25N3O11 | M.Wt | 555.49 |
| Solubility | DMSO : 25 mg/mL (45.01 mM; Need ultrasonic) | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.8002 mL | 9.0011 mL | 18.0021 mL |
| 5 mM | 360 μL | 1.8002 mL | 3.6004 mL |
| 10 mM | 180 μL | 900.1 μL | 1.8002 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.50% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 7 reference(s) in Google Scholar.)