SNOTRAP (SNO trapping by triaryl phosphine) is a chemical probe used for detecting S-nitrosylated ( SNO ) proteins. SNOTRAP selectively converts S-nitrosylated cysteines in proteins into stable disulfide-iminophosphorane intermediates, which are then enriched with streptavidin and detected by nanoLC-MS/MS. SNOTRAP enables global enrichment and label-free quantification of SNO proteins and their modification sites in complex biological samples such as mouse brain tissues. SNOTRAP can be applied to studies related to nitrosative stress-associated diseases, including neurodegenerative diseases, cancer, and multiple sclerosis [1] [2].
In Vitro, SNOTRAP (1.5 mM; 2 h) efficiently labels endogenous S-nitrosylated proteins in mouse brain tissue (cortex, striatum, hippocampus) lysates, enabling the identification of 1181 SNO proteins (1714 SNO sites) with a specificity of 98%[1].
References:
[1]. Yang H, et al. An improved sulfur-nitroso-proteome strategy for global profiling of sulfur-nitrosylated proteins and sulfur-nitrosylation sites in mice. Journal of chromatography. A. 2023 Aug 30;1705:464162.
[2]. Yang H, et al. Mechanistic insight into female predominance in Alzheimer's disease based on aberrant protein S-nitrosylation of C3. Science advances. 2022 Dec 14;8(50):eade0764.
In Vitro, SNOTRAP (1.5 mM; 2 h) efficiently labels endogenous S-nitrosylated proteins in mouse brain tissue (cortex, striatum, hippocampus) lysates, enabling the identification of 1181 SNO proteins (1714 SNO sites) with a specificity of 98%[1].
References:
[1]. Yang H, et al. An improved sulfur-nitroso-proteome strategy for global profiling of sulfur-nitrosylated proteins and sulfur-nitrosylation sites in mice. Journal of chromatography. A. 2023 Aug 30;1705:464162.
[2]. Yang H, et al. Mechanistic insight into female predominance in Alzheimer's disease based on aberrant protein S-nitrosylation of C3. Science advances. 2022 Dec 14;8(50):eade0764.