GLPBio

Methylprednisolone

Catalog No.: GC15505Purity:>98.00%Synonyms: Medesone, Medrol, Noretona, NSC 19987, U-7532
COASDSData Sheet
Methylprednisolone is a corticosteroid that interacts with the glucocorticoid receptor and inhibits cortisol secretion with an IC50 of 1.7ng/ml.

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Methylprednisolone
Cas No.: 83-43-2
10mM (in 1mL DMSO)In Stock
$30.00
1
100mgIn Stock
$68.00
1
250mgIn Stock
$108.00
1
500mgIn Stock
$135.00
1
1000mgIn Stock
$195.00
1
21gIn Stock
$2,561.00
1
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Description

Methylprednisolone is a corticosteroid that interacts with the glucocorticoid receptor and inhibits cortisol secretion with an IC50 of 1.7ng/ml [1]. Methylprednisolone exerts anti-inflammatory effects by inhibiting NF-kappaB and can be utilized in the treatment of tuberculosis and COVID-19 [2].

Methylprednisolone (0.5µM, 5µM, 30µM, or 50µM) could reduce HOG cell proliferation and promote HOG death in a dose-dependent manner. Methylprednisolone also decreased the expression of MBP, a marker for early mature oligodendrocytes, and inhibited myelin regeneration [3].

Methylprednisolone (25mg/kg) treatment significantly ameliorated l Aβ1-42-induced cognitive deficits in Alzheimer's disease mice as well as reduced microglia hyperactivation. Synaptic function-related genes could be upregulated by Methylprednisolone, while immune response-related genes were suppressed by Methylprednisolone. Methylprednisolone alleviated cognitive dysfunction by improving synaptic function and suppressing immune and inflammatory processes [4].

References:
[1]. Lew KH, Ludwig EA, Milad MA, Donovan K, Middleton E Jr, Ferry JJ, Jusko WJ. Gender-based effects on methylprednisolone pharmacokinetics and pharmacodynamics. Clin Pharmacol Ther. 1993 Oct;54(4):402-14. doi: 10.1038/clpt.1993.167. PMID: 8222483; PMCID: PMC4207261.

[2]. Li A, He Y, Yang C, Lu N, Bao J, Gao S, Hosyanto FF, He X, Fu H, Yan H, Ding N, Xu L. Methylprednisolone Promotes Mycobacterium smegmatis Survival in Macrophages through NF-κB/DUSP1 Pathway. Microorganisms. 2023 Mar 16;11(3):768. doi: 10.3390/microorganisms11030768. PMID: 36985341; PMCID: PMC10058212.

[3]. GÓmez-Pinedo U, MatÍas-Guiu JA, Ojeda-Hernandez D, de la Fuente-Martin S, Kamal OM, Benito-Martin MS, Selma-Calvo B, Montero-Escribano P, MatÍas-Guiu J. In Vitro Effects of Methylprednisolone over Oligodendroglial Cells: Foresight to Future Cell Therapies. Cells. 2023 May 30;12(11):1515. doi: 10.3390/cells12111515. PMID: 37296635; PMCID: PMC10252523.

[4]. Sun Y, Li J, A N, Li Z, Zhong W, Chen L, Liu S, Zhang B, Zhu Z, Li X. Methylprednisolone alleviates cognitive functions through the regulation of neuroinflammation in Alzheimer's disease. Front Immunol. 2023 May 1;14:1192940. doi: 10.3389/fimmu.2023.1192940. PMID: 37197654; PMCID: PMC10183590.

Protocol

Cell experiment [1]:

Cell lines

Human oligodendroglioma cell HOG

Preparation Method

HOG cells were cultured in Dulbecco's Modified Eagle's Medium at 37 °C with 5% CO2. After 24 hours of cell incubation, the medium was changed to a medium containing different doses (0.5µM, 5µM, 30µM, or 50µM) of methylprednisolone for 3, 5, and 10 days.

Reaction Conditions

0.5µM, 5µM, 30µM, or 50µM methylprednisolone for 3, 5, and 10 days

Applications

Methylprednisolone reduced the proliferation of HOG cells by reducing cell viability in a dose-dependent manner. HOG's death was also enhanced by methylprednisolone. In addition, methylprednisolone reduced the expression of the mature oligodendrocyte marker MBP at an early stage and inhibited myelin regeneration
Animal experiment [2]:

Animal models

6-week-old male C57/BL6 mice (weight 18-22 g)

Preparation Method

Methylprednisolone was dissolved in saline. Mice were intragastrically administrated with methylprednisolone (25mg/kg) for 2-14 days.

Dosage form

25mg/kg, intragastrically administration

Applications

Methylprednisolone alleviated cognitive dysfunctions and inhibited the excessive activation of microglia in Aβ1-42-induced mice. Synaptic function-related genes were upregulated by Methylprednisolone, whereas immune response-related genes were suppressed by Methylprednisolone.

References:

[1]. GÓmez-Pinedo U, MatÍas-Guiu JA, Ojeda-Hernandez D, de la Fuente-Martin S, Kamal OM, Benito-Martin MS, Selma-Calvo B, Montero-Escribano P, MatÍas-Guiu J. In Vitro Effects of Methylprednisolone over Oligodendroglial Cells: Foresight to Future Cell Therapies. Cells. 2023 May 30;12(11):1515. doi: 10.3390/cells12111515. PMID: 37296635; PMCID: PMC10252523.
[2]. Sun Y, Li J, A N, Li Z, Zhong W, Chen L, Liu S, Zhang B, Zhu Z, Li X. Methylprednisolone alleviates cognitive functions through the regulation of neuroinflammation in Alzheimer's disease. Front Immunol. 2023 May 1;14:1192940. doi: 10.3389/fimmu.2023.1192940. PMID: 37197654; PMCID: PMC10183590.

Product Documents

Purity:>98.00%Appearance:A solid
COASDSData Sheet

Chemical Properties

CAS No.
83-43-2
Synonyms
Medesone, Medrol, Noretona, NSC 19987, U-7532
Chemical Name
(6S,8S,9S,10R,11S,13S,14S,17R)-11,17-dihydroxy-17-(2-hydroxyacetyl)-6,10,13-trimethyl-7,8,9,11,12,14,15,16-octahydro-6H-cyclopenta[a]phenanthren-3-one
SMILES
CC1CC2C3CCC(C3(CC(C2C4(C1=CC(=O)C=C4)C)O)C)(C(=O)CO)O
Molecular Formula
C22H30O5
Molecular Weight
374.48
Solubility
≥ 15.35 mg/mL in DMSO, ≥ 9.5 mg/mL in EtOH with ultrasonic
Storage
4°C, protect from light
General tips
Select a suitable solvent according to the product solubility data. Once prepared, aliquot the stock solution to avoid repeated freeze-thaw cycles.
Use stock solutions within 6 months when stored at -80°C or within 1 month when stored at -20°C.
To improve solubility, warm the tube to 37°C and sonicate it for an appropriate period.
Shipping Condition
Evaluation samples are shipped with blue ice. Other available sizes are shipped at room temperature or with blue ice upon request.

CalculatorsMolarity / Dilution / Molecular Weight / Unit Conversion / In Vivo Formulation / Solubility

g/mol

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