GLPBio

Papain (≥800u/mg)

Catalog No.: GC33829Purity:803u/mg
COASDSData Sheet
Papain (≥800u/mg)is a cysteine endopeptidase with strong proteolytic activity against a broad range of protein substrates.

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Papain (≥800u/mg)
Cas No.: 9001-73-4
100mgIn Stock
$67.00
1
500mgIn Stock
$116.00
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1gIn Stock
$184.00
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5gIn Stock
$368.00
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Citations

1 publicationsPublications from researchers using this product

Description

Papain (≥800u/mg) is a cysteine endopeptidase with strong proteolytic activity against a broad range of protein substrates[1]. The Cys-25 region of the active site of Papain attacks the carbonyl carbon in the peptide backbone, releasing the amino-terminal region and thereby causing protein dissociation, and Papain exhibits optimal activity within a temperature range of 50°C to 59°C and a pH range of 4.5 to 6.7[2]. Papain has been widely used in the food processing industry to increase protein solubility, reduce the content of allergenic proteins in grains, and decrease insoluble protein aggregates[3].

In vitro, Papain significantly inhibited the viability of K562 cells, with IC50 values of 386µM and 483µM at 24 and 48 hours of treatment, respectively[4].

In vivo, Papain treatment via intratracheal injection (50μl; 0.2IU/μl) twice within one week induced emphysema in mice, resulting in increased functional residual capacity and elastic fiber deposition[5]. Intranasal administration of 20μg/day of Papain on days 0-2 and 7-10 induced allergic rhinosinusitis in C57BL/6 mice, leading to eosinophilia in nasal lavage fluid and goblet cell hyperplasia[6]. Three consecutive days of intratracheal injection of 100μg/day of Papain caused lung inflammation in mice and damaged the lung epithelial cells[7].

References:

[1] Tacias-Pascacio V G, Morellon-Sterling R, Castaneda-Valbuena D, et al. Immobilization of papain: A review[J]. International Journal of Biological Macromolecules, 2021, 188: 94-113.

[2] Babalola B A, Akinwande A I, Otunba A A, et al. Therapeutic benefits of Carica papaya: A review on its pharmacological activities and characterization of papain[J]. Arabian Journal of Chemistry, 2024, 17.

[3] Fernández-Lucas J, Castañeda D, Hormigo D. New trends for a classical enzyme: Papain, a biotechnological success story in the food industry[J]. Trends in Food Science & Technology, 2017, 68: 91-101.  

[4] Meriç N, Kar E, Kar F. Papain induces Mitochondria-mediated apoptosis and modulates inflammatory signaling in leukemic cells[J]. Molecular Biology Reports, 2026, 53(1): 334.

[5] Machado M N, Mazzoli-Rocha F, Casquilho N V, et al. Bone marrow-derived mononuclear cell therapy in papain-induced experimental pulmonary emphysema[J]. Frontiers in Physiology, 2018, 9: 121.

[6] Tharakan A, Dobzanski A, London Jr N R, et al. Characterization of a novel, papain‐inducible murine model of eosinophilic rhinosinusitis[C]//International forum of allergy & rhinology. 2018, 8(4): 513-521.

[7] Morita H, Arae K, Unno H, et al. An interleukin-33-mast cell-interleukin-2 axis suppresses papain-induced allergic inflammation by promoting regulatory T cell numbers[J]. Immunity, 2015, 43(1): 175-186.

Protocol

Cell experiment [1]:

Cell lines

 K562 cells

Preparation Method

 K562 cells were cultured in RPMI-1640 medium supplemented with 10% fetal bovine serum (FBS), 2mM L-glutamine, and 1% PSA (containing penicillin 10000units/ml, streptomycin 10000µg/ml, and amphotericin B 25µg/ml) at 37°C in an incubator with 5% CO2. Cells (5×103/well) were seeded in 96-well plates and cultured at 37°C in 5% CO2 overnight and then treated with different concentrations of Papain (0, 12.5, 25, 50, and 100μM) for 48h, cell viability was assessed.

Reaction Conditions

 0, 12.5, 25, 50, and 100μM; 48h

Applications

 Papain treatment reduced cell viability of K562 cells in a dose-dependent manner.
Animal experiment [2]:

Animal models

 BALB/c mice

Preparation Method

BALB/c mice (20-25g) were housed in a specific-pathogen-free environment under a 12h/12h light-dark cycle and fed rodent diet ad libitum. Mice were intratracheally injected with 50μl of sterile saline solution (0.9% NaCl) containing 10IU of Papain once a week for two weeks. Lung tissues of mice were studied 21 days after the first saline or Papain administration.

Dosage form

10U/50μl (50μl); once a week; 2 weeks; intratracheal injection

Applications

Papain treatment induced lung mechanical harm, oxidative stress, larger alveoli in mice.

References:

[1] Meriç N, Kar E, Kar F. Papain induces Mitochondria-mediated apoptosis and modulates inflammatory signaling in leukemic cells[J]. Molecular Biology Reports, 2026, 53(1): 334.

[2] Machado M N, da Silva Figueirôa S F, Mazzoli-Rocha F, et al. Papain-induced experimental pulmonary emphysema in male and female mice[J]. Respiratory Physiology & Neurobiology, 2014, 200: 90-96.

Product Documents

Biological Activity:803u/mgAppearance:A solid
COASDSData Sheet

Chemical Properties

CAS No.
9001-73-4
SMILES
[Papain]
Solubility
Water : 50 mg/mL (Need ultrasonic);DMSO : 5 mg/mL (Need ultrasonic)
Storage
Store at 2-8°C
Shipping Condition
Evaluation samples are shipped with blue ice. Other available sizes are shipped at room temperature or with blue ice upon request.

CalculatorsMolarity / Dilution / Molecular Weight / Unit Conversion

g/mol

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