Papain (≥800u/mg) |
Catalog No.GC33829
One-Click Copy Product Info
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Papain (≥800u/mg)is a cysteine endopeptidase with strong proteolytic activity against a broad range of protein substrates.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 9001-73-4
Sample solution is provided at 25 µL, 10mM.
Papain (≥800u/mg) is a cysteine endopeptidase with strong proteolytic activity against a broad range of protein substrates[1]. The Cys-25 region of the active site of Papain attacks the carbonyl carbon in the peptide backbone, releasing the amino-terminal region and thereby causing protein dissociation, and Papain exhibits optimal activity within a temperature range of 50°C to 59°C and a pH range of 4.5 to 6.7[2]. Papain has been widely used in the food processing industry to increase protein solubility, reduce the content of allergenic proteins in grains, and decrease insoluble protein aggregates[3].
In vitro, Papain significantly inhibited the viability of K562 cells, with IC50 values of 386µM and 483µM at 24 and 48 hours of treatment, respectively[4].
In vivo, Papain treatment via intratracheal injection (50μl; 0.2IU/μl) twice within one week induced emphysema in mice, resulting in increased functional residual capacity and elastic fiber deposition[5]. Intranasal administration of 20μg/day of Papain on days 0-2 and 7-10 induced allergic rhinosinusitis in C57BL/6 mice, leading to eosinophilia in nasal lavage fluid and goblet cell hyperplasia[6]. Three consecutive days of intratracheal injection of 100μg/day of Papain caused lung inflammation in mice and damaged the lung epithelial cells[7].
References:
[1] Tacias-Pascacio V G, Morellon-Sterling R, Castaneda-Valbuena D, et al. Immobilization of papain: A review[J]. International Journal of Biological Macromolecules, 2021, 188: 94-113.
[2] Babalola B A, Akinwande A I, Otunba A A, et al. Therapeutic benefits of Carica papaya: A review on its pharmacological activities and characterization of papain[J]. Arabian Journal of Chemistry, 2024, 17.
[3] Fernández-Lucas J, Castañeda D, Hormigo D. New trends for a classical enzyme: Papain, a biotechnological success story in the food industry[J]. Trends in Food Science & Technology, 2017, 68: 91-101.
[4] Meriç N, Kar E, Kar F. Papain induces Mitochondria-mediated apoptosis and modulates inflammatory signaling in leukemic cells[J]. Molecular Biology Reports, 2026, 53(1): 334.
[5] Machado M N, Mazzoli-Rocha F, Casquilho N V, et al. Bone marrow-derived mononuclear cell therapy in papain-induced experimental pulmonary emphysema[J]. Frontiers in Physiology, 2018, 9: 121.
[6] Tharakan A, Dobzanski A, London Jr N R, et al. Characterization of a novel, papain‐inducible murine model of eosinophilic rhinosinusitis[C]//International forum of allergy & rhinology. 2018, 8(4): 513-521.
[7] Morita H, Arae K, Unno H, et al. An interleukin-33-mast cell-interleukin-2 axis suppresses papain-induced allergic inflammation by promoting regulatory T cell numbers[J]. Immunity, 2015, 43(1): 175-186.
| Cell experiment [1]: | |
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Cell lines |
K562 cells |
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Preparation Method |
K562 cells were cultured in RPMI-1640 medium supplemented with 10% fetal bovine serum (FBS), 2mM L-glutamine, and 1% PSA (containing penicillin 10000units/ml, streptomycin 10000µg/ml, and amphotericin B 25µg/ml) at 37°C in an incubator with 5% CO2. Cells (5×103/well) were seeded in 96-well plates and cultured at 37°C in 5% CO2 overnight and then treated with different concentrations of Papain (0, 12.5, 25, 50, and 100μM) for 48h, cell viability was assessed. |
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Reaction Conditions |
0, 12.5, 25, 50, and 100μM; 48h |
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Applications |
Papain treatment reduced cell viability of K562 cells in a dose-dependent manner. |
| Animal experiment [2]: | |
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Animal models |
BALB/c mice |
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Preparation Method |
BALB/c mice (20-25g) were housed in a specific-pathogen-free environment under a 12h/12h light-dark cycle and fed rodent diet ad libitum. Mice were intratracheally injected with 50μl of sterile saline solution (0.9% NaCl) containing 10IU of Papain once a week for two weeks. Lung tissues of mice were studied 21 days after the first saline or Papain administration. |
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Dosage form |
10U/50μl (50μl); once a week; 2 weeks; intratracheal injection |
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Applications |
Papain treatment induced lung mechanical harm, oxidative stress, larger alveoli in mice. |
References: [1] Meriç N, Kar E, Kar F. Papain induces Mitochondria-mediated apoptosis and modulates inflammatory signaling in leukemic cells[J]. Molecular Biology Reports, 2026, 53(1): 334. [2] Machado M N, da Silva Figueirôa S F, Mazzoli-Rocha F, et al. Papain-induced experimental pulmonary emphysema in male and female mice[J]. Respiratory Physiology & Neurobiology, 2014, 200: 90-96. | |
| Cas No. | 9001-73-4 | SDF | |
| Canonical SMILES | [Papain] | ||
| Formula | M.Wt | ||
| Solubility | Water : 50 mg/mL (Need ultrasonic);DMSO : 5 mg/mL (Need ultrasonic) | Storage | Store at 2-8°C |
| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
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Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Biological Activity: 803u/mg Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 38 reference(s) in Google Scholar.)