LDAO (Synonyms: Dimethyldodecylamine N-oxide, Lauramine Oxide, Lauryldimethylamine Oxide, N-Lauryldimethylamine N-oxide) |
Catalog No.GC44046
One-Click Copy Product Info
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LDAO (Lauryl Dimethylamine Oxide) es un detergente no iónico/zwitterionic que se puede utilizar para solubilizar y estabilizar las proteínas de la membrana
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 1643-20-5
Sample solution is provided at 25 µL, 10mM.
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LDAO (Lauryl Dimethylamine Oxide) es un detergente no iónico/zwitterionic que se puede utilizar para solubilizar y estabilizar las proteínas de la membrana[1]. La capacidad de descontaminación leve de LDAO le permite interrumpir eficazmente la estructura bicapa lipídica mientras mantiene la conformación nativa de la proteína[2]. LDAO se utiliza a menudo para solubilizar proteínas transmembrana (como los receptores acoplados a proteínas G) en membranas celulares bacterianas o de mamíferos mientras se mantiene su actividad funcional[3]. LDAO puede afectar indirectamente la actividad de la H+-ATPase al disolver la estructura de la membrana mitocondrial, regulando así el proceso de hidrólisis ATP [4]. LDAO añadido a las reacciones químicas de clic sin cobre aumentó significativamente la eficiencia de unión de las variantes de CRM197 a los Streptococcus pneumoniae polysaccharides[5]. La adición de LDAO al medio de crecimiento de Rhodovulum sulfidophilum resultó en cambios drásticos en la biosíntesis de carotenoid[6].
References:
[1] Veldhuis G, Gabellieri E, Poolman B, et al. The effect of the detergent-shell on the structure of a membrane-protein[J]. Mechanics of the mannitol transporter from Escherichia coli, 111.
[2] Orwick‐Rydmark M, Arnold T, Linke D. The use of detergents to purify membrane proteins[J]. Current protocols in protein science, 2016, 84(1): 4.8. 1-4.8. 35.
[3] Marcovich I. Structural studies of enzymes: a bacterial flippase and a human kinase[J]. 2020.
[4] Vazquez-Laslop N, Dreyfus G. Mitochondrial H+-ATPase activation by an amine oxide detergent[J]. Journal of Biological Chemistry, 1986, 261(17): 7807-7810.
[5] Bautista L, Pill-Pepe L, Kapoor N, et al. Addition of lauryldimethylamine N-oxide (LDAO) to a copper-free click chemistry reaction improves the conjugation efficiency of a cell-free generated CRM197 variant to clinically important Streptococcus pneumoniae serotypes[J]. ACS omega, 2022, 7(39): 34921-34928.
[6] Hagemann G E, Sturgis J N, Wagner E, et al. The effects of the detergent LDAO on the carotenoid metabolism and growth of Rhodovulum sulfidophilum[J]. Microbiological research, 1996, 151(4): 421-426.
| Cas No. | 1643-20-5 | SDF | |
| Sinónimos | Dimethyldodecylamine N-oxide, Lauramine Oxide, Lauryldimethylamine Oxide, N-Lauryldimethylamine N-oxide | ||
| Canonical SMILES | CCCCCCCCCCCC[N+](C)([O-])C | ||
| Formula | C14H31NO | M.Wt | 229.4 |
| Solubility | DMF: 0.3 mg/ml,DMSO: 0.1 mg/ml,Ethanol: 15 mg/ml,PBS (pH 7.2): 0.1 mg/ml | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 4.3592 mL | 21.796 mL | 43.592 mL |
| 5 mM | 871.8 μL | 4.3592 mL | 8.7184 mL |
| 10 mM | 435.9 μL | 2.1796 mL | 4.3592 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 11 reference(s) in Google Scholar.)