GLPBio

MeOSuc-Ala-Ala-Pro-Val-AMC

Catalog No.: GC44157Purity:>98.00%Synonyms: I 1270, MeOSucAAPVAMC, Methoxy SuccinylAlaAlaProValAMC
COASDSData Sheet
MeOSuc-Ala-Ala-Pro-Val-AMC es un sustrato fluorogénico para leucocitos humanos y elastasa pancreÁtica porcina (Km: 362 μM, Ex=380 nm, Em=460 nm).

Los productos son exclusivamente para uso en investigación. No aptos para uso humano. No vendemos a pacientes.

MeOSuc-Ala-Ala-Pro-Val-AMC
Cas No.: 72252-90-5
10mM (in 1mL DMSO)In Stock
$93.00
1
1mgIn Stock
$24.00
1
5mgIn Stock
$67.00
1
10mgIn Stock
$96.00
1
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    641, 529–536 (2025)
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    632, 686–694 (2024)
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    618, 1017–1023 (2023)
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    Nature
    610, 366–372 (2022)
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    Cell
    187(9):2288-2304 (2024)
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    Cell
    183(7):1867-1883 (2020)
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    35, 97–116 (2025)
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    34, 683–706 (2024)
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    33, 904–922 (2023)
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    31, 1291–1307 (2021)

Descripción (Description)

MeOSuc-Ala-Ala-Pro-Val-AMC is a highly sensitive and specific fluorescent substrate (excitation/emission wavelengths = 355-380/440-460nm) that can be used to determine the activity of neutrophil elastase. MeOSuc-Ala-Ala-Pro-Val-AMC can be used in research related to inflammatory lung diseases and cancer[1-4].

References:
[1] Zhang J, Friberg IM, Kift-Morgan A, et al. Machine-learning algorithms define pathogen-specific local immune fingerprints in peritoneal dialysis patients with bacterial infections. Kidney Int. 2017 Jul;92(1):179-191.
[2] Eming SA, Smola-Hess S, Kurschat P, et al. A novel property of povidon-iodine: inhibition of excessive protease levels in chronic non-healing wounds. J Invest Dermatol. 2006 Dec;126(12):2731-3.
[3] Evans, Vikki. Synthesis of Optical Probes for the Visualization of Human Neutrophil Elastase. The University of Edinburgh (United Kingdom) ProQuest Dissertations & Theses. 2019. 28046287.
[4] Ivanov D, Emonet C, Foata F, et al. A serpin from the gut bacterium Bifidobacterium longum inhibits eukaryotic elastase-like serine proteases. J Biol Chem. 2006 Jun 23;281(25):17246-17252.

Protocolo (Protocol)

MeOSuc-Ala-Ala-Pro-Val-AMC Fluorescence Method for Determining Elastase Activity[1]
This protocol is adapted from research data and provided for reference only. Adjustments may be necessary based on specific experimental requirements.
1. Reagent Preparation: Dissolve MeOSuc-Ala-Ala-Pro-Val-AMC in dimethyl sulfoxide (DMSO) to prepare a stock solution, and store at -40°C. Before use, dilute the DMSO stock solution approximately 100-fold into the reaction buffer to achieve a final concentration of 400μM MeOSuc-Ala-Ala-Pro-Val-AMC, or adjust according to the requirements of kinetic experiments.

2. Preparation of test solution: Collect the supernatant after cell lysis, or prepare the working concentration of elastase (e.g., porcine pancreatic elastase; PPE; 50nM), and equilibrate at 37°C for 10 minutes before use for subsequent detection.


3. Activity Assay:Rapidly mix 50μL of 400μM MeOSuc-Ala-Ala-Pro-Val-AMC with 50μL of the test solution in a 96-well clear bottom plate. Immediately record the fluorescence change over time using a fluorescence microplate reader (excitation/emission wavelengths = 355-380/440-460nm).
Precautions:
(1) For your safety and health, please wear a lab coat and disposable gloves during operation.

References:
[1] Ivanov D, Emonet C, Foata F, et al. A serpin from the gut bacterium Bifidobacterium longum inhibits eukaryotic elastase-like serine proteases. J Biol Chem. 2006 Jun 23;281(25):17246-17252.

Documentos del producto (Product Documents)

Purity:>98.00%Appearance:A solid
COASDSData Sheet

Propiedades químicas (Chemical Properties)

N.º CAS
72252-90-5
Sinónimos
I 1270, MeOSucAAPVAMC, Methoxy SuccinylAlaAlaProValAMC
SMILES
COC(CCC(N[C@H](C(N[C@H](C(N1CCC[C@H]1C(N[C@@H](C(C)C)C(NC2=CC=C(C(C)=CC(O3)=O)C3=C2)=O)=O)=O)C)=O)C)=O)=O
Fórmula molecular
C31H41N5O9
Peso molecular
627.7
Solubilidad
DMF: 20 mg/ml,DMF:PBS(pH 7.2)(1:1): 0.5 mg/ml,DMSO: 16 mg/ml
Almacenamiento
Store at -20°C
Consejos generales
Seleccione un disolvente adecuado según los datos de solubilidad del producto. Una vez preparada, divida la solución madre en alícuotas para evitar ciclos repetidos de congelación y descongelación.
Utilice las soluciones madre en un plazo de 6 meses si se conservan a -80°C, o de 1 mes si se conservan a -20°C.
Para mejorar la solubilidad, caliente el tubo a 37°C y soníquelo durante un periodo adecuado.
Condiciones de envío
Las muestras de evaluación se envían con acumuladores de frío. Los demás tamaños se envían a temperatura ambiente o, previa solicitud, con acumuladores de frío.

Calculadoras (Calculators)Molaridad / Dilución / Peso molecular / Conversión de unidades / Formulación in vivo / Solubilidad

g/mol

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