GLPBio

OD 36 hydrochloride

Catalog No.: GC50517Purity:>99.50%
COASDSData Sheet
El clorhidrato de OD 36 es un inhibidor de RIPK2 con un IC50 de 5,3 nM.

Los productos son exclusivamente para uso en investigación. No aptos para uso humano. No vendemos a pacientes.

OD 36 hydrochloride
Cas No.: 1638644-62-8
1mgIn Stock
$29.00
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5mgIn Stock
$70.00
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10mgIn Stock
$103.00
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25mgIn Stock
$173.00
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50mgIn Stock
$261.00
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    641, 529–536 (2025)
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    610, 366–372 (2022)
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    Cell
    187(9):2288-2304 (2024)
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    183(7):1867-1883 (2020)
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    35, 97–116 (2025)
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Descripción (Description)

OD 36 hydrochloride is a potent inhibitor of RIPK2 kinase with an IC50 value of 5.3nM [1]. OD 36 hydrochloride mimics the interaction between the ATP adenine ring and the hinge region of RIPK2 KD, and utilizes the interaction between the cyclic linker group and the hydrophobic posterior pocket as well as the ribose binding region, thereby enhancing the affinity and selectivity for RIPK2[2]. OD 36 hydrochloride has been widely used in mouse peritonitis models to alleviate inflammation[3].

In vitro, OD 36 hydrochloride treatment (0.5μM) for 21 days significantly inhibited the osteogenic and chondrogenic differentiation induced by activin A in ATDC5 cells expressing mutant R206H ALK2 [4]. The 5.3nM OD 36 hydrochloride treatment for 24 hours significantly inhibited the invasion and metastasis of K7M2 cells promoted by macrophages[5].

In vivo, OD 36 hydrochloride treatment via intraperitoneal injection at a single dose of 6.25mg/kg can reduce the cell infiltration in a muramyl dipeptide (MDP)-induced peritonitis mouse model [6].

References:
[1] Shen S, Lu C, Ling T, et al. Current advances on RIPK2 and its inhibitors in pathological processes: a comprehensive review[J]. Frontiers in Molecular Neuroscience, 2025, 18: 1492807.
[2] Pham A T, Ghilardi A F, Sun L. Recent advances in the development of RIPK2 modulators for the treatment of inflammatory diseases[J]. Frontiers in pharmacology, 2023, 14: 1127722.
[3] You J, Wang Y, Chen H, et al. RIPK2: a promising target for cancer treatment[J]. Frontiers in pharmacology, 2023, 14: 1192970.
[4] Sánchez Duffhues G, Williams E, Benderitter P, et al. Development of Macrocycle Kinase Inhibitors for ALK2 Using Fibrodysplasia Ossificans Progressiva Derived Endothelial Cells[J]. Journal of Bone and Mineral Research Plus, 2019, 3(11): e10230.
[5] Maloney C, Kallis M P, Edelman M, et al. Gefitinib inhibits invasion and metastasis of osteosarcoma via inhibition of macrophage receptor interacting serine-threonine kinase 2[J]. Molecular cancer therapeutics, 2020, 19(6): 1340-1350.
[6] Tigno-Aranjuez J T, Benderitter P, Rombouts F, et al. In vivo inhibition of RIPK2 kinase alleviates inflammatory disease[J]. Journal of Biological Chemistry, 2014, 289(43): 29651-29664.

Protocolo (Protocol)

Cell experiment [1]:

Cell lines

ATDC5 cells

Preparation Method

ATDC5 cells were transduced with the same titer of adenoviral particles in the presence of Polybrene (4mg/ml), encoding for either the ALK2wt‐HA or ALK2R206H‐HA. ATDC5 cells were trypsinized and washed once with PBS. There were 3×105 cells counted per micromass, and resuspended in 10μl of culture medium. Very carefully, 100‐μl drops were deposited in the center of the well in a 24‐wells plate and placed in the incubator for 2 hours. Next 500μl of DMEM‐F12 5% fetal bovine serum (FBS) containing 1× ITS were carefully added to the wells. After 24 hours, the medium was replaced by DMEM‐F12 5% FBS containing 1× ITS, supplemented with activin A (100ng/ml), and DMSO, LDN‐193189, OD 36 hydrochloride, or OD52 (0.5μM). The cells were incubated for 21 days before further analysis, refreshing the medium every 5 days. Finally, the cells were washed and pictures acquired using a microscope with 10× magnification.

Reaction Conditions

0.5μM; 21 days

Applications

OD 36 hydrochloride treatment significantly inhibited the osteogenic and chondrogenic differentiation induced by activin A in ATDC5 cells expressing mutant R206H ALK2.
Animal experiment [2]:

Animal models

C57BL/6 mice

Preparation Method

C57BL/6 mice were housed in an air-conditioned room with a 12h light/dark cycle and received standard laboratory rat chow and tap water. Rats were allowed 7 days to adjust to their new environment. C57BL/6 mice were given vehicle or 6.25mg/kg of Gefitinib or OD 36 hydrochloride intraperitoneal in a 500μl volume. Thirty minutes later, mice were injected with 150μg of MDP intraperitoneal. Four hours later, mice were sacrificed and a peritoneal lavage using 5ml of PBS was performed.

Dosage form

6.25mg/kg for once; i.p.

Applications

OD 36 hydrochloride treatment reduced the cell infiltration in a muramyl dipeptide (MDP)-induced peritonitis mouse model.

References:
[1] Sánchez Duffhues G, Williams E, Benderitter P, et al. Development of Macrocycle Kinase Inhibitors for ALK2 Using Fibrodysplasia Ossificans Progressiva Derived Endothelial Cells[J]. Journal of Bone and Mineral Research Plus, 2019, 3(11): e10230.
[2] Tigno-Aranjuez J T, Benderitter P, Rombouts F, et al. In vivo inhibition of RIPK2 kinase alleviates inflammatory disease[J]. Journal of Biological Chemistry, 2014, 289(43): 29651-29664.

Documentos del producto (Product Documents)

Purity:>99.50%Appearance:A solid
COASDSData Sheet

Propiedades químicas (Chemical Properties)

N.º CAS
1638644-62-8
SMILES
ClC1=CC(C2=C3N=C(NCCOCCO4)C=CN3N=C2)=CC4=C1.Cl
Fórmula molecular
C16H15ClN4O2.HCl
Peso molecular
367.23
Solubilidad
DMSO : 33.33 mg/mL (100.76 mM; ultrasonic and warming and heat to 60°C)
Almacenamiento
Store at 2-8°C
Consejos generales
Seleccione un disolvente adecuado según los datos de solubilidad del producto. Una vez preparada, divida la solución madre en alícuotas para evitar ciclos repetidos de congelación y descongelación.
Utilice las soluciones madre en un plazo de 6 meses si se conservan a -80°C, o de 1 mes si se conservan a -20°C.
Para mejorar la solubilidad, caliente el tubo a 37°C y soníquelo durante un periodo adecuado.
Condiciones de envío
Las muestras de evaluación se envían con acumuladores de frío. Los demás tamaños se envían a temperatura ambiente o, previa solicitud, con acumuladores de frío.

Calculadoras (Calculators)Molaridad / Dilución / Peso molecular / Conversión de unidades / Formulación in vivo / Solubilidad

g/mol

Reviews

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