Sphingosine-13C2-d2 (d18:1) is intended for use as an analytical standard for the quantification of sphingosine (d18:1) by GC- or LC-MS. Sphingosine (d18:1) is formed primarily from the breakdown of ceramide.1 Sphingosine (d18:1) inhibits protein kinase C and phosphatidic acid phosphohydrolase, whereas it activates phospholipase D and diacylglycerol (DAG) kinase.1 Phosphorylation of sphingosine (d18:1) by sphingosine kinases 1 and 2 (SPHK1, SPHK2) produces sphingosine-1-phosphate, a potent bioactive lipid that exhibits a broad spectrum of biological activities including cell proliferation, survival, migration, cytoskeletal organization, and morphogenesis.2,3,4
1.Hannun, Y.A., Luberto, C., and Argraves, K.M.Enzymes of sphingolipid metabolism: From modular to integrative signalingBiochemistry40(16)4893-4903(2001) 2.Takuwa, Y., Takuwa, N., and Sugimoto, N.The Edg family G protein-coupled receptors for lysophospholipids: Their signaling properties and biological activitiesJ. Biochem.131(6)767-771(2002) 3.Ishii, I., Fukushima, N., Ye, X., et al.Lysophospholipid receptors: Signaling and biologyAnnu. Rev. Biochem.73321-354(2004) 4.Kluk, M.J., and Hla, T.Signaling of sphingosine-1-phosphate via the S1P/EDG-family of G-protein-coupled receptorsBiochim. Biophys. Acta1582(1-3)72-80(2002)