Unit definition:One unit will convert one micromole of Xanthine to Uric acid per min at pH 7.5 at 37℃.
Isoelectric point: 4.0.
Michaelis constant: 1.4×10 M (Xanthine).
Optimum pH: 7.0~ 7.5.
Optimum temperature: 55℃.
pH Stability :6.0~9.5(30℃,16hr).
Thermal stability: < 55℃ (pH 7.5, 20min).
Inhibitors: Ag ,Hg.
Reddish to brown amorphous powder. It is soluble in water. Microbial extraction. The main inhibitors are Ag+, Hg2+, Cd2+ and urea, cyanide, phosphate and chloride ions, and the main stabilizers are salicylate, cysteine, histamine and vilenoate.
It is an enzyme that catalyzes the production of uric acid from xanthine and hypoxanthine, and it is often used as a tool enzyme. For the determination of xanthine, hypoxanthine, inosine, guanine, guanosine, etc