24α-methyl Cholesterol (Synonyms: Campesterol,NSC 224330) |
| Catalog No.GC13431 |
24α-methyl Cholesterol is a plant sterol with cholesterol lowering and anticarcinogenic effects.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 474-62-4
Sample solution is provided at 25 µL, 10mM.
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24α-methyl Cholesterol is an agonist of liver X receptors (LXR).
Liver X receptors (LXRs) are ligand-dependent transcription factors involved in the transcriptional control of lipid metabolism. LXRs function as nuclear cholesterol sensors that are activated by elevated intracellular cholesterol levels in multiple cell types. Activation of LXRs induces the expression of various genes involved in cholesterol absorption, transport, efflux, and excretion. In addition to their function in lipid metabolism, LXRs have also been found to modulate immune and inflammatory responses in macrophages. LXRα is highly expressed in the liver and at lower levels in the adrenal glands, adipose, intestine, lung, macrophages, and kidney, and LXRβ is ubiquitously expressed [2].
24α-methyl Cholesterol was a phytosterol found in vegetables, nuts, fruits, and seeds that competitively inhibited the absorption of intestinal cholesterol and decreased the transcription of genes important for cholesterol metabolism [3]. 24α-methyl Cholesterol acted as an agonist at liver X receptors (LXR) and suppressed the proliferation of prostate and breast cancer cells [1].
References:
[1] Chuu C P, Kokontis J M, Hiipakka R A, et al. Modulation of liver X receptor signaling as novel therapy for prostate cancer[J]. Journal of biomedical science, 2007, 14(5): 543-553.
[2] Zelcer N, Tontonoz P. Liver X receptors as integrators of metabolic and inflammatory signaling[J]. The Journal of clinical investigation, 2006, 116(3): 607-614.
[3] Segura R, Javierre C, Lizarraga M A, et al. Other relevant components of nuts: phytosterols, folate and minerals[J]. British Journal of Nutrition, 2006, 96(S2): S36-S44.
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Cell experiment: |
The effect of campesterol on the viable cell number was assessed by XTT assay. HUVECs were treated with various concentrations (10, 20, 30, 40 and 50 μg/mL) of campesterol and incubated at 37 °C in a humidified incubator for 24 h, a XTT working solution was added to each well. The cells were incubated at 37 °C for 2 h and the optical density was measured using a microplate reader at 450 nm[1]. |
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References: [1]. Choi JM, et al. Identification of campesterol from Chrysanthemum coronarium L. and its antiangiogenic activities. Phytother Res. 2007 Oct;21(10):954-9. |
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| Cas No. | 474-62-4 | SDF | |
| Synonyms | Campesterol,NSC 224330 | ||
| Chemical Name | (3β,24R)-ergost-5-en-3-ol | ||
| Canonical SMILES | O[C@H](C1)CC[C@@]2(C)C1=CC[C@]3([H])[C@]2([H])CC[C@@]4(C)[C@@]3([H])CC[C@]4([H])[C@H](C)CC[C@@H](C)C(C)C | ||
| Formula | C28H48O | M.Wt | 400.7 |
| Solubility | ≤0.25mg/ml in ethanol; 1mg/ml in dimethyl formamide; 0.8mg/ml in Water (ultrasonic and warming and heat) | Storage | Store at 2-8°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.4956 mL | 12.4782 mL | 24.9563 mL |
| 5 mM | 0.4991 mL | 2.4956 mL | 4.9913 mL |
| 10 mM | 0.2496 mL | 1.2478 mL | 2.4956 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
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μL
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Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 40 reference(s) in Google Scholar.)
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