IF 15
PMID: 39500898 ↗4-Methylumbelliferyl-β-D-glucuronide hydrate (MUG)
Catalog No.: GC30519Purity:>99.50%
4-Methylumbelliferyl-β-D-glucuronide hydrate (MUG) is a fluorogenic substrat (Λex=362 nm , Λem=445 nm).
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 881005-91-0
10mM (in 1mL DMSO)In Stock
$40.00
1
25mgIn Stock
$36.00
1
50mgIn Stock
$58.00
1
100mgIn Stock
$92.00
1
| Pack Size | Price | Availability | Quantity | Action |
|---|---|---|---|---|
| 10mM (in 1mL DMSO) | $40.00 | In Stock | 1 | |
| 25mg | $36.00 | In Stock | 1 | |
| 50mg | $58.00 | In Stock | 1 | |
| 100mg | $92.00 | In Stock | 1 |
Tel: (909) 407-4943 / (909) 689-7124Email: sales@glpbio.com
Citations
2 publicationsPublications from researchers using this productDescription
4-Methylumbelliferyl-β-D-glucuronide hydrate is a fluorogenic substrat (λex=362 nm , λem=445 nm).
4-Methylumbelliferyl-β-D-glucuronide hydrate is a fluorogenic substrat with λex=362 nm and λem=445 nm. The half-saturation constants, Km, that are calculated for sewage samples are close to the Km values calculated based on experimental plots presented previously for freshwaters[1].
[1]. George I, et al. Use of beta-D-galactosidase and beta-D-glucuronidase activities for quantitative detection of totaland fecal coliforms in wastewater. Can J Microbiol. 2001 Jul;47(7):670-5.
Protocol
Kinase experiment: | GLUase activity of E. coli cells is measured. Three millilitres of 4-Methylumbelliferyl-β-D-glucuronide hydrate (MUGlu) solution (55 mg of hydrated MUGlu and 20 mL of Triton X-100 in 50 mL of sterile water) is added to each flask (final concentration:165 mg/L). The incubation temperature is 44°C. One hundred microlitres of 2 M NaOH solution is added to each 2.9-mL aliquot to obtain a pH>10 before the fluorescence measurement[1]. |
References: [1]. George I, et al. Use of beta-D-galactosidase and beta-D-glucuronidase activities for quantitative detection of totaland fecal coliforms in wastewater. Can J Microbiol. 2001 Jul;47(7):670-5. | |
Product Documents
Purity:>99.50%Appearance:A solid
Chemical Properties
CAS No.
881005-91-0
SMILES
O[C@H]([C@H]([C@@H]([C@@H](C(O)=O)O1)O)O)[C@@H]1OC2=CC=C(C(O3)=C2)C(C)=CC3=O.O
Molecular Formula
C16H18O10
Molecular Weight
370.31
Solubility
DMSO : 150 mg/mL (405.07 mM);Water : 5.2 mg/mL (14.04 mM)
Storage
Store at 4°C, protect from light
General tips
Select a suitable solvent according to the product solubility data. Once prepared, aliquot the stock solution to avoid repeated freeze-thaw cycles.
Use stock solutions within 6 months when stored at -80°C or within 1 month when stored at -20°C.
To improve solubility, warm the tube to 37°C and sonicate it for an appropriate period.
Use stock solutions within 6 months when stored at -80°C or within 1 month when stored at -20°C.
To improve solubility, warm the tube to 37°C and sonicate it for an appropriate period.
Shipping Condition
Evaluation samples are shipped with blue ice. Other available sizes are shipped at room temperature or with blue ice upon request.
CalculatorsMolarity / Dilution / Molecular Weight / Unit Conversion / In Vivo Formulation / Solubility
g/mol
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