AF488 azide is a fluorescent dye that can be used to determine the reactivity of immobilized DIBO groups. AF488 azide exhibits the λ em and λ ex wavelength of 520 nm and 470 nm, respectively [1] [2].
In Vitro, AF488 azide contains an Azide group and can undergo copper-catalyzed azide-alkyne cycloaddition reaction (CuAAc) with molecules containing Alkyne groups. It can also undergo strain-promoted alkyne-azide cycloaddition (SPAAC) reactions with molecules containing DBCO or BCN groups[1][2].
References:
[1]. Ruff SM, et al. clickECM: Development of a cell-derived extracellular matrix with azide functionalities. Acta Biomater. 2017 Apr 1;52:159-170.
[2]. Chakraborty S, Hu S Y, Wu S H, et al. The interaction affinity between vascular cell adhesion molecule-1 (VCAM-1) and very late antigen-4 (VLA-4) analyzed by quantitative FRET[J]. PLoS One, 2015, 10(3): e0121399.
In Vitro, AF488 azide contains an Azide group and can undergo copper-catalyzed azide-alkyne cycloaddition reaction (CuAAc) with molecules containing Alkyne groups. It can also undergo strain-promoted alkyne-azide cycloaddition (SPAAC) reactions with molecules containing DBCO or BCN groups[1][2].
References:
[1]. Ruff SM, et al. clickECM: Development of a cell-derived extracellular matrix with azide functionalities. Acta Biomater. 2017 Apr 1;52:159-170.
[2]. Chakraborty S, Hu S Y, Wu S H, et al. The interaction affinity between vascular cell adhesion molecule-1 (VCAM-1) and very late antigen-4 (VLA-4) analyzed by quantitative FRET[J]. PLoS One, 2015, 10(3): e0121399.