Dyes, Stains & Indicators

FerroOrange (Fe 2+ indicator) is a fluorescent probe for detecting ferrous ions Fe 2+ in living cells. It is not suitable for dead cells. The maximum excitation/emission wavelengths are 543nm/580nm.

BODIPY 505/515-8-C3-COOH is a green fluorescing derivative, as a fluorescent dye for imaging lipid droplets in nannochloropsis. BODIPY 505/515-8-C3-COOH can be used for the research of flow cytometric high-throughput screening and cell sorting.

OG 488, acid, a fluorescent pH indicator, has many applications in biochemistry and neurosciences.

NFQ1 is a non-fluorescent quencher (absorption wavelength: 474 nm), and is used for a new type of One Sample Fluorescence Resonance Energy Transfer (OS-FRET) method.

H-D-Val-Leu-Lys-pNA . 2 HCl is a colorimetric substrate for the detection of protease activity, particularly plasmin.

H-Gln-AMC is a fluorogenic substrate commenly used to measure the activity of glutaminyl cyclase (QC) enzymes with K m value of 51 ± 3µM at pH 8.0 and k cat value of 5.4 ± 0.1s -1 .

Suc-Ala-Ala-Pro-Ile-pNA (Suc-AAPI-pNA) is a synthetic peptide compound used as a substrate for detecting and studying the activity of various proteases. Under the action of enzymes, Suc-Ala-Ala-Pro-Ile-pNA is hydrolyzed, releasing yellow p-nitroaniline (pNA). The change in absorbance at 400-410 nm is measured by colorimetry to reflect enzyme activity.

Suc-Ala-Phe-Lys-AMC is a highly sensitive fluorogenic substrate.

Tos-Gly-Pro-Arg-AMC . HCl is a fluorescent substrate for thrombin, which is widely used to detect serine protease activity. Its excitation and emission wavelengths are 380nm and 460nm, respectively.

Z-Ala-Ala-Asn-AMC (Cbz-Ala-Ala-Asn-AMC) is a synthetic fluorogenic peptide substrate that releases highly fluorescent AMC upon cleavage by proteases (Ex/Em: 380nm/430nm), which is widely used for the detection of asparaginyl endopeptidase (Legumain) activity.

Z-Gly-Pro-AMC functions as a fluorogenic substrate that is susceptible to hydrolysis by prolyl endopeptidase, resulting in the production of intensely fluorescent 7-amido-4-methylcoumarin. (Λex=380 nm, Λem=465 nm).

Amplex Red is utilized to measure hydrogen peroxide concentrations or peroxidase activities.

Mag-Fura-2 AM is a fluorescent dye which is useful for measuring changes in cytosolic Mg 2+ with E x /E m =336/503nm.

Merocyanin 540 is a fluorescent membrane probe that selectively stains the membranes of various electrically excitable cells but not non-excitable cells. Its maximum excitation wavelength Ex is 540nm and its maximum emission wavelength Em is 580nm.

SYBR Green I for Real Time PCR, 100x is a convenient, highly sensitive, high-quality nucleic acid fluorescent dye suitable for fluorescent staining quantification in qPCR, isothermal amplification or gene chips. It can also be used for fluorescent staining of DNA or RNA during agarose gel or polyacrylamide gel electrophoresis. The maximum absorption wavelength of SYBR Green I is about 497nm, and the maximum emission wavelength is about 520nm.

Biotin-XX Tyramide Reagent is a biotin derivative for enzyme-mediated tyramide signal amplification (TSA).

FITC (Fluorescein isothiocyanate) is the most commonly used green fluorescent dye derivative in biological research and is widely used in flow cytometry experiments to stain proteins, cell nuclei and subnuclear particles. The maximum excitation/emission wavelengths of FITC are 495/519 nm respectively.

Biotin-tyramide is a biotin-labeled tyramine derivative and is the core reagent of the Tyramide Signal Amplification (TSA) technology.

SYBR Green I Gel Staining Solution, 10000x is an unsymmetrical cyanine dye developed for sensitive detection of nucleic acids in electrophoretic gels.

MOCAc-PLGL(Dpa)AR is a fluorescent substrate specifically designed for the detection of matrix metalloproteinases (MMPs), which is suitable for the activity of MMP-2, MMP-7 and MMP-9.

TRITC Phalloidin is a fluorescein derivative of Phalloidin, which can specifically binds rabbit skeletal muscle F-actin with a K d value of 20nM.

9-Amino-6-chloro-2-methoxyacridine (ACMA) is a multifunctional, cell-permeable fluorescent probe and bioactive molecule that can be utilized as a DNA intercalator and a pH/membrane potential-sensitive probe.

FITC-Dextran (MW 4000) (FD4) is a large molecule of dextran (MW ~4000) attached to a fluorescein isothiocyanate tag, which makes it fluorescent (Ex=495 nm; Em=525 nm).

Lyso-Tracker Red is an eosinophilic red fluorescent probe that can penetrate the cell membrane at neutral pH and can be used for lysosome-specific fluorescent staining of living cells and tracing of acidic organelles. The maximum excitation and emission wavelengths of Lyso-Tracker Red are 577/590 nm.