8-Azaadenosine (Synonyms: NSC 72961) |
رقم الكتالوجGC50588
One-Click Copy Product Info
|
8-Azaadenosine هو مثبط قوي لـ ADAR1 ومثبط تحرير A-to-I
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 10299-44-2
Sample solution is provided at 25 µL, 10mM.
_1-3.$$$39978408@51.jpg');)
_1-9.$$$38538795@65.jpg');)
_1-9.$$$39112701@51.jpg');)
_1-7.$$$37316672@70.jpg');)
_366-372.$$$36198801@70.jpg');)
.$$$38565142@65.jpg');)
_1867-1883.$$$33248023@67.jpg');)
_eads6055.$$$39977546@45.jpg');)
_eadm9805.$$$40080571@45.jpg');)
_eadj3020.$$$39666821@45.jpg');)
_1-20.$$$39757301@28.jpg');)
_1-24.$$$38898113@28.jpg');)
_273-287.$$$36806353@46.jpg');)
_1-16.$$$37156877@44.jpg');)
_1-19.$$$37460805@44.jpg');)
_1291-1307.$$$34518654@46.jpg');)
8-Azaadenosine is a potent adenosine deaminase acting on RNA1 (ADAR1) inhibitor that reduces ADAR1-mediated A-to-I editing activity and prevents self-renewal of the K562 leukemic cell line[1]. ADAR1-mediated A-to-I editing is an important pathway involved in the progression of various cancers, including thyroid cancer. 8-Azaadenosine inhibits proliferation, 3D growth, invasion, and migration in thyroid cancer cells[2]. 8-Azaadenosine targets ADAR activity only and does not affect ADAR1 mRNA levels[2].
In vitro, 8-Azaadenosine (0.1-2µM; 1-6d) decreased cell viability/proliferation in a dose-dependent manner in TPC1 and Cal62 cells. 8-Azaadenosine (1, 2µM; 72h) inhibited A-to-I editing activity of both TPC1 and Cal62 cells. 8-Azaadenosine (1, 2µM; 16h) reduced 3D growth and 2D invasion and migration in TPC1 and Cal62 cells[2].
References:
[1] Zipeto MA, Court AC, Sadarangani A, et al. ADAR1 Activation Drives Leukemia Stem Cell Self-Renewal by Impairing Let-7 Biogenesis. Cell Stem Cell. 2016;19(2):177-191.
[2] Ramírez-Moya, Julia et al. “ADAR1-mediated RNA editing is a novel oncogenic process in thyroid cancer and regulates miR-200 activity.” Oncogene vol. 39,18 (2020): 3738-3753.
| Cell experiment [1]: | |
Cell lines | TPC1 and Cal62 |
Preparation Method | 8-Azaadenosine dissolved in water was added to the cell culture medium. MTT assays at the indicated time points at 100nM, 500nM, 1µM, and 2µM 8-Azaadenosine concentration. |
Reaction Conditions | 100nM, 500nM, 1µM, and 2µM; 1-6d |
Applications | 8-Azaadenosine decreased cell viability/proliferation in a dose-dependent manner in TPC1 and Cal62 cells. |
References: | |
| Cas No. | 10299-44-2 | SDF | |
| المرادفات | NSC 72961 | ||
| Canonical SMILES | NC1=NC=NC2=C1N=NN2[C@@H]1O[C@H](CO)[C@@H](O)[C@H]1O | ||
| Formula | C9H12N6O4 | M.Wt | 268.23 |
| الذوبان | DMSO : 250 mg/mL (932.04 mM; Need ultrasonic); H2O : 3.33 mg/mL (12.41 mM; ultrasonic and warming and heat to 60°C) | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
||
| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 3.7281 mL | 18.6407 mL | 37.2814 mL |
| 5 mM | 745.6 μL | 3.7281 mL | 7.4563 mL |
| 10 mM | 372.8 μL | 1.8641 mL | 3.7281 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.50% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 14 reference(s) in Google Scholar.)