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Palmitic acid

Catalog No.: GN10676Purity:>98.00%Synonyms: C16:0, Cetylic Acid
COASDSData Sheet

حمض دهني مشبع طويل السلسلة

المنتجات مخصصة للاستخدام البحثي فقط، وليست للاستخدام البشري. نحن لا نبيع للمرضى.

Palmitic acid
Cas No.: 57-10-3
10mM (in 1mL DMSO)In Stock
$46.00
1
10gIn Stock
$38.00
1
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المراجع (Citations)

8 publicationsمنشورات الباحثين الذين استخدموا هذا المنتج
IF 11

الوصف (Description)

Palmitic acid is a 16-carbon long-chain saturated fatty acid that can post-translationally modify proteins [1]. Palmitic acid can reduce the expressions of p-STAT3, p-JAK2, n-cadherin and vimentin, inhibit the nuclear localization of p-STAT3, and thereby suppress tumor cell metastasis and proliferation[2]. Palmitic acid has been widely used to enhance the signal transduction induced by TLR4 and to strengthen the inflammatory response[3].

In vitro, Palmitic acid treatment for 72 hours significantly inhibited the viability of Ishikawa cells and ECC-1 cells, with IC50 values of 348.2µM and 187.3µM, respectively[4]. Treatment with 300μM Palmitic acid for 24 hours significantly induced the production of reactive oxygen species (ROS) in HepG2 cells and led to a decrease in mitochondrial membrane potential[5]. 150μM of Palmitic acid treatment for 12 hours significantly promoted the maturation of monocyte-derived dendritic cells (MoDCs) and the secretion of IL-1β[6].

In vivo, Palmitic acid treatment via intraperitoneal injection at a dose of 50mg/kg/day for 18 days significantly inhibited the tumor growth in the SGC-7901 xenograft mouse model, without affecting body weight[7]. A single intraperitoneal injection of 30μmol (50μl) of Palmitic acid reduced the locomotor activity of mice within 2 hours, induced anxiety-like behaviors in mice, and simultaneously increased serotonin metabolism mediated by the amygdala[8].

References:
[1] Fatima S, Hu X, Gong R H, et al. Palmitic acid is an intracellular signaling molecule involved in disease development[J]. Cellular and Molecular Life Sciences, 2019, 76(13): 2547-2557.
[2] Wang X, Zhang C, Bao N. Molecular mechanism of palmitic acid and its derivatives in tumor progression[J]. Frontiers in oncology, 2023, 13: 1224125.
[3] Korbecki J, Bajdak-Rusinek K. The effect of palmitic acid on inflammatory response in macrophages: an overview of molecular mechanisms[J]. Inflammation Research, 2019, 68(11): 915-932.
[4] Zhao Z, Wang J, Kong W, et al. Palmitic acid exerts anti-tumorigenic activities by modulating cellular stress and lipid droplet formation in endometrial cancer[J]. Biomolecules, 2024, 14(5): 601.
[5] Alnahdi A, John A, Raza H. Augmentation of glucotoxicity, oxidative stress, apoptosis and mitochondrial dysfunction in HepG2 cells by palmitic acid[J]. Nutrients, 2019, 11(9): 1979.
[6] Nicholas D A, Zhang K, Hung C, et al. Palmitic acid is a toll-like receptor 4 ligand that induces human dendritic cell secretion of IL-1β[J]. PloS one, 2017, 12(5): e0176793.
[7] Yu X, Peng W, Wang Y, et al. Palmitic acid inhibits the growth and metastasis of gastric cancer by blocking the STAT3 signaling pathway[J]. Cancers, 2023, 15(2): 388.
[8] Moon M L, Joesting J J, Lawson M A, et al. The saturated fatty acid, palmitic acid, induces anxiety-like behavior in mice[J]. Metabolism, 2014, 63(9): 1131-1140.

البروتوكول (Protocol)

Cell experiment [1]:

Cell lines

Ishikawa cells

Preparation Method

Preparation of Palmitic acid working solution: Add 78μl of anhydrous ethanol to 1mg Palmitic acid and dissolve to 50mM stock solution (ultrasonic heating for 3min; 37°C); Take an appropriate amount of Fatty acid free BSA Add DMEM culture medium to prepare a 10% BSA solution (100mg/ml). Take 5μl of Palmitic acid stock solution (50mM), add 100μl of BSA solution (10%), mix well (ultrasonic heating for 3min; 37°C), add 895μl of DMEM culture medium, mix well (ultrasonic heating for 3min; 37°C) to prepare 0.25mM Palmitic acid working solution.

Ishikawa cells were cultured in DMEM/F-12 medium enriched with 10% fetal bovine serum (FBS) and 1% antimycotic-antibiotic at 37°C in 5% CO2/atmosphere. Cells were seeded at a density of 2×104 cells/ml in the 96-well plates and allowed to adhere in a 5% CO2 incubator at 37°C for 24h. The cells were treated with different concentrations of Palmitic acid (0, 0.1, 1, 10, 50, 100, and 250μM) for 96h. The cell viability was evaluated.

Reaction Conditions

0, 0.1, 1, 10, 50, 100, and 250μM; 96h

Applications

Palmitic acid treatment significantly inhibited cell viability of Ishikawa cells in a dose-dependent manner.
Animal experiment [2]:

Animal models

Male BALB/c nude mice

Preparation Method

Male BALB/c nude mice (6-week-old; 18-22g) were housed in a room with controlled temperature (21-23°C), humidity (55%-60%) and lighting (12h light/dark cycle) and were supplied with water ad libitum. Approximately 5×106 SGC-7901 cells were injected into the flanks of the nude mice to establish a human gastric cancer syngeneic s.c. xenograft model. When the cancer volume reached approximately 50mm3, Palmitic acid was administered via i.p. injections once daily at the designed doses (50mg/kg or vehicle) for 18 days. Cancer growth and mouse body weights were measured every other day throughout the experiment. Cancer volumes (mm3) were calculated as 0.5×length (mm)×width2(mm).

Dosage form

50mg/kg/day; 18 days; i.p.

Applications

Palmitic acid treatment significantly inhibited the tumor growth in the SGC-7901 xenograft mouse model, without affecting body weight.

References:
[1] Zhao Z, Wang J, Kong W, et al. Palmitic acid exerts anti-tumorigenic activities by modulating cellular stress and lipid droplet formation in endometrial cancer[J]. Biomolecules, 2024, 14(5): 601.
[2] Yu X, Peng W, Wang Y, et al. Palmitic acid inhibits the growth and metastasis of gastric cancer by blocking the STAT3 signaling pathway[J]. Cancers, 2023, 15(2): 388.

وثائق المنتج (Product Documents)

Purity:>98.00%Appearance:A solid
COASDSData Sheet

الخصائص الكيميائية (Chemical Properties)

رقم CAS
57-10-3
المرادفات
C16:0, Cetylic Acid
الاسم الكيميائي
hexadecanoic acid
SMILES
CCCCCCCCCCCCCCCC(=O)O
الصيغة الجزيئية
C16H32O2
الوزن الجزيئي
256.42
الذوبانية
≥ 51mg/mL in DMSO; ≥ 51mg/mL in Ethanol
التخزين
Store at -20°C,protect from light
شروط الشحن
تُشحن عينات التقييم مع عبوات تبريد. وتُشحن الأحجام الأخرى المتاحة في درجة حرارة الغرفة، أو مع عبوات تبريد عند الطلب.

الحاسبات (Calculators)المولارية / التخفيف / الوزن الجزيئي / تحويل الوحدات / تركيبة داخل الجسم الحي / الذوبانية

g/mol

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