Procyanidin B2 (Synonyms: (-)-Epicatechin-(4β→8)-(-)-epicatechin, Proanthocyanidin B2, Procyanidol B2) |
| رقم الكتالوجGN10599 |
Procyanidin B2 (PB2), an antioxidant from the proanthocyanidin flavonoid polyphenol group, is found in plants like grape seeds, berries, cocoa, and tea, offering pharmacological benefits including anti-oxidative and anti-inflammatory effects.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 29106-49-8
Sample solution is provided at 25 µL, 10mM.
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Procyanidin B2 (PB2), an antioxidant from the proanthocyanidin flavonoid polyphenol group, is found in plants like grape seeds, berries, cocoa, and tea, offering pharmacological benefits including anti-oxidative and anti-inflammatory effects [1-3].
Procyanidin B2, when administered at concentrations ranging from 0 to 10 μM for 24 h, suppresses the secretion of sFlt-1 from primary placental tissues andendothelial cells [4]. Additionally, at concentrations of 1 to 2.5 μM, Procyanidin B2 enhances the angiogenic function, survival, and migration of Endothelial Progenitor Cells (EPCs) exposed to high glucose (HG) conditions [5].
Procyanidin B2 (PB2), administered intravenously at doses of 0.5, 1, or 2 mg/kg, contributes to the treatment of sepsis-induced Acute Lung Injury (ALI) by regulating cytokine storms and mitigating inflammation through modulation of the TLR4/NF-κB, PI3K/Akt, Hippo, and Rho signaling pathways [6]. Furthermore, Procyanidin B2 (50 mg/kg/day) administered orally for 6 weeks attenuates liver injury and hepatocyte pyroptosis in mice exposed to nicotine [7].
References:
[1]. Chen J, Zhong K, et,al. Procyanidin B2: A promising multi-functional food-derived pigment for human diseases. Food Chem. 2023 Sep 15;420:136101. doi: 10.1016/j.foodchem.2023.136101. Epub 2023 Apr 7. Erratum in: Food Chem. 2024 Feb 1;433:137359. PMID: 37059021.
[2]. Zhang JQ, Gao BW, et,al. Critical Role of FoxO1 in Granulosa Cell Apoptosis Caused by Oxidative Stress and Protective Effects of Grape Seed Procyanidin B2. Oxid Med Cell Longev. 2016;2016:6147345. doi: 10.1155/2016/6147345. Epub 2016 Jan 19. PMID: 27057282; PMCID: PMC4745910.
[3]. Kopustinskiene DM, Savickas A, et,al. Direct effects of (-)-epicatechin and procyanidin B2 on the respiration of rat heart mitochondria. Biomed Res Int. 2015;2015:232836. doi: 10.1155/2015/232836. Epub 2015 Feb 24. PMID: 25811024; PMCID: PMC4354975.
[4]. Liu L, Wang R, et,al. Procyanidin B2 ameliorates endothelial dysfunction and impaired angiogenesis via the Nrf2/PPARγ/sFlt-1 axis in preeclampsia. Pharmacol Res. 2022 Mar;177:106127. doi: 10.1016/j.phrs.2022.106127. Epub 2022 Feb 10. PMID: 35150862.
[5]. Fan J, Liu H, et,al. Procyanidin B2 improves endothelial progenitor cell function and promotes wound healing in diabetic mice via activating Nrf2. J Cell Mol Med. 2021 Jan;25(2):652-665. doi: 10.1111/jcmm.16111. Epub 2020 Nov 20. PMID: 33215883; PMCID: PMC7812287.
[6]. Kim GO, Park DH, et,al. Procyanidin B2 Attenuates Sepsis-Induced Acute Lung Injury via Regulating Hippo/Rho/PI3K/NF-κB Signaling Pathway. Int J Mol Sci. 2023 Apr 27;24(9):7930. doi: 10.3390/ijms24097930. PMID: 37175637; PMCID: PMC10177954.
[7]. Liu J, Yao Q, et,al. Procyanidin B2 Attenuates Nicotine-Induced Hepatocyte Pyroptosis through a PPARγ-Dependent Mechanism. Nutrients. 2022 Apr22;14(9):1756. doi: 10.3390/nu14091756. PMID: 35565726; PMCID: PMC9103831.
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Cell experiment [1]: |
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Cell lines |
HUVECs( Human Umbilical Vein Endothelial Cells，HUVEC) |
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Preparation method |
HUVECs were treated with different concentrations of procyanidin B2 (0, 2.5, 5, and 10 μM) for 24 h. |
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Reaction Conditions |
0-10 μM; 24 h |
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Applications |
Procyanidin B2 inhibits sFlt-1 secretion from HUVEC in a concentration-dependent manner. |
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Animal experiment [2]: |
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Animal models |
Male C57BL/6 mice (27 g; 6–7 weeks old) |
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Preparation method |
LPS was injected into the mice via intraperitoneal injection at a dose of 5 mg/kg with 0.2% DMSO. One hour and 12 h after the LPS injection, the mice were given intravenous doses of Procyanidin B2 (0.5, 1, or 2 mg/kg). |
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Dosage form |
0.5, 1, or 2 mg/kg; i.v |
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Applications |
Procyanidin B2 Alleviated Acute Lung Injury Induced by Intraperitoneal LPS. |
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References: [1]. Liu L, Wang R, et,al. Procyanidin B2 ameliorates endothelial dysfunction and impaired angiogenesis via the Nrf2/PPARγ/sFlt-1 axis in preeclampsia. Pharmacol Res. 2022 Mar;177:106127. doi: 10.1016/j.phrs.2022.106127. Epub 2022 Feb 10. PMID: 35150862. [2]. Kim GO, Park DH, et,al.Procyanidin B2 Attenuates Sepsis-Induced Acute Lung Injury via Regulating Hippo/Rho/PI3K/NF-κB Signaling Pathway. Int J Mol Sci. 2023 Apr 27;24(9):7930. doi: 10.3390/ijms24097930. PMID: 37175637; PMCID: PMC10177954. |
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| Cas No. | 29106-49-8 | SDF | |
| المرادفات | (-)-Epicatechin-(4β→8)-(-)-epicatechin, Proanthocyanidin B2, Procyanidol B2 | ||
| Chemical Name | (2R,3R)-2-(3,4-dihydroxyphenyl)-8-[(2R,3R,4R)-2-(3,4-dihydroxyphenyl)-3,5,7-trihydroxy-3,4-dihydro-2H-chromen-4-yl]-3,4-dihydro-2H-chromene-3,5,7-triol | ||
| Canonical SMILES | C1C(C(OC2=C1C(=CC(=C2C3C(C(OC4=CC(=CC(=C34)O)O)C5=CC(=C(C=C5)O)O)O)O)O)C6=CC(=C(C=C6)O)O)O | ||
| Formula | C30H26O12 | M.Wt | 578.52 |
| الذوبان | DMF: 30 mg/mL,DMSO: 30 mg/mL,Ethanol: 30 mg/mL,PBS (pH 7.2): 10 mg/mL | Storage | Store at -20℃ |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.7285 mL | 8.6427 mL | 17.2855 mL |
| 5 mM | 345.7 μL | 1.7285 mL | 3.4571 mL |
| 10 mM | 172.9 μL | 864.3 μL | 1.7285 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
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μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)
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