GLPBio

RGDS peptide

Catalog No.: GC16385Purity:>98.50%Synonyms: Fibronectin Inhibitor, HArgGlyAspSerOH
COASDSData Sheet
RGDS الببتيد هو تسلسل ارتباط إنتيجرين يثبط وظيفة مستقبلات إنتجرين.

المنتجات مخصصة للاستخدام البحثي فقط، وليست للاستخدام البشري. نحن لا نبيع للمرضى.

RGDS peptide
Cas No.: 91037-65-9
10mM (in 1mL DMSO)In Stock
$24.00
1
5mgIn Stock
$22.00
1
10mgIn Stock
$38.00
1
50mgIn Stock
$123.00
1
100mgIn Stock
$221.00
1
Tel: (909) 407-4943 / (909) 689-7124Email: sales@glpbio.com

المراجع (Citations)

لا تتوفر حاليًا مراجع لهذا المنتج. فيما يلي مجموعة مختارة من المنشورات التي تستشهد بمنتجات GlpBio.
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    632, 686–694 (2024)
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    618, 1017–1023 (2023)
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    610, 366–372 (2022)
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    187(9):2288-2304 (2024)
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    183(7):1867-1883 (2020)
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الوصف (Description)

RGDS peptide contains the Arg-Gly-Asp (RGD) motif that mediates the interaction between extracellular matrix proteins and cell integrins and is widely used to probe integrin function[1]. RGDS peptide demonstrates significant antibacterial potential as an antibiotic adjuvant[2]. RGDS peptide covalently functionalized ND (NDCO-RGDS) can also be a tumor-targeting carrier of VEGF-siRNA[3].

In vitro, treatment of osteoblast-like cells with 0.2mM RGDS peptide for three days completely blocked apoptosis induced by staurosporine, the Ca2+·Pi ion pair, and sodium nitroprusside[4]. Following 500μg/mL RGDS peptide treatment for 2, 6, 16, and 24h, SK-MEL-110 cells internalized RGDS peptide in a time-dependent manner and exhibited marked anti-proliferative and pro-apoptotic effects independent of any extracellular anti-adhesive action[5].

In vivo, a single intraperitoneal injection of RGDS peptide 1h before lipopolysaccharide (LPS) challenge (1, 2.5, or 5mg/kg) dose-dependently suppressed the LPS-induced increases in neutrophil and macrophage counts, total protein content, TNF-α and macrophage inflammatory protein (MIP)-2 levels, and matrix metalloproteinase-9 activity in bronchoalveolar lavage fluid[6]. In Balb/c mice, a single intraperitoneal injection of RGDS peptide (5mg/kg) 30min before LPS (50μg/kg) and D-GalN (800mg/kg) markedly reduced hepatic injury and mortality[7].

References:
[1] Ortega-Velázquez R, Díez-Marqués ML, Ruiz-Torres MP, et al. Arg-Gly-Asp-Ser (RGDS) peptide stimulates transforming growth factor beta1 transcription and secretion through integrin activation. FASEB J. 2003;17(11):1529-1531.
[2] Feng Y, Xie W, Wan Z, et al. Harnessing RGDS peptides to regulate bacterial-host interfaces for targeted antimicrobial therapy. J Control Release. 2025;384:113922.
[3] Cui C , Wang Y , Zhao W , et al. RGDS covalently surfaced nanodiamond as a tumor targeting carrier of VEGF-siRNA: synthesis, characterization and bioassay. J Mater Chem B. 2015;3(48):9260-9268.
[4] Grigoriou V, Shapiro IM, Cavalcanti-Adam EA, et al. Apoptosis and survival of osteoblast-like cells are regulated by surface attachment. J Biol Chem. 2005;280(3):1733-1739.
[5] Aguzzi MS, Fortugno P, Giampietri C, et al. Intracellular targets of RGDS peptide in melanoma cells. Mol Cancer. 2010;9:84.
[6] Moon C, Han JR, Park HJ, et al. Synthetic RGDS peptide attenuates lipopolysaccharide-induced pulmonary inflammation by inhibiting integrin signaled MAP kinase pathways. Respir Res. 2009;10(1):18.
[7] Yin X, Gong X, Jiang R, et al. Synthetic RGDS peptide attenuated lipopolysaccharide/D-galactosamine-induced fulminant hepatic failure in mice. J Gastroenterol Hepatol. 2014;29(6):1308-1315.

البروتوكول (Protocol)

Cell experiment [1]:

Cell lines

Osteoblast-like cells

Preparation Method

To evaluate cell death, osteoblast-like cells were plated on experimental (RGDS peptide) and control (Arg-Gly-Glu-Ser (RGES)) substrates in 12- or 24-well plates at a density of 50,000/well or 25,000/well, respectively. After 3 days in culture in complete media, the osteoblasts were incubated overnight with sublethal doses of staurosporine (0.1 and 0.5μM), the Ca2+ (2.4 and 2.9mM) and Pi (3mM) ion pair, or sodium nitroprusside (0.5 and 0.1mM).

Reaction Conditions

0.2mM; 3 days

Applications

Cells were grown on the RGDS peptide substrate and treated with 0.1 and 0.5μM staurosporine; no significant cell killing was observed. In contrast, staurosporine killed osteoblasts maintained on the RGES surface in a dose-dependent manner. Although 3mM Pi and 2.4mM Ca2+ did not kill osteoblasts grown on the RGDS peptide surface, there was a significant increase in cell death on the RGES surface. A further increase in cell death was apparent when osteoblasts were treated with 3mM Pi and 2.9mM Ca2+. Again, there was no significant increase in death among osteoblasts maintained on the RGDS peptide surface. The effect of the Nitric Oxide (NO) donor sodium nitroprusside on osteoblast apoptosis was similar to that of the Ca2+·Pi ion pair and staurosporine. At concentrations of 0.1 and 0.5mM, sodium nitroprusside killed 45 and 65% of osteoblasts cultured on the RGES surface, respectively. These concentrations of sodium nitroprusside failed to kill osteoblasts on the RGDS peptide surface.

Animal experiment [2]:

Animal models

Male BALB/C mice

Preparation Method

Animals were anesthetized with a mixture of ketamine and xylazine (45mg/kg and 8mg/kg; i.p.; respectively). Test solution (30μl) containing lipopolysaccharide (LPS) (1.5mg/kg) was placed posterior to the throat and aspirated into the lungs. Control mice were administered sterile saline (0.9% NaCl). Animals were administered with RGDS peptide or Arg-Gly-Glu-Ser (RGES) peptide (1, 2.5, or 5mg/kg; i.p.) one hour before LPS treatment and sacrificed 4h post-LPS. Animals were also administered RGDS peptide or RGES peptide (5mg/kg; i.p.) once at different time points (1h before or 2h after LPS treatment) and sacrificed 24h post-LPS.

Dosage form

1, 2.5, or 5mg/kg; i.p.

Applications

A pretreatment with RGDS peptide inhibited LPS-induced increases in neutrophil and macrophage numbers, total protein levels, and TNF-α and macrophage inflammatory protein (MIP)-2 levels, and matrix metalloproteinase-9 activity in bronchoalveolar lavage (BAL) fluid in a dose-dependent manner at 4 or 24h post-LPS treatment. RGDS peptide inhibited LPS-induced phosphorylation of focal adhesion kinase and mitogen-activated protein (MAP) kinases, including ERK, JNK, and p38 MAP kinase, in lung tissue. Importantly, the inhibition of the inflammatory responses and the kinase pathways was still evident when this peptide was administered 2h after LPS treatment.

References:
[1] Grigoriou V, Shapiro IM, Cavalcanti-Adam EA, et al. Apoptosis and survival of osteoblast-like cells are regulated by surface attachment. J Biol Chem. 2005;280(3):1733-1739.
[2] Moon C, Han JR, Park HJ, et al. Synthetic RGDS peptide attenuates lipopolysaccharide-induced pulmonary inflammation by inhibiting integrin signaled MAP kinase pathways. Respir Res. 2009;10(1):18.

وثائق المنتج (Product Documents)

Purity:>98.50%Appearance:A solid
COASDSData Sheet

الخصائص الكيميائية (Chemical Properties)

رقم CAS
91037-65-9
المرادفات
Fibronectin Inhibitor, HArgGlyAspSerOH
الاسم الكيميائي
(6R,12S,15R)-1,1,6-triamino-12-(carboxymethyl)-15-(hydroxymethyl)-7,10,13-trioxo-2,8,11,14-tetraazahexadec-1-en-16-oic acid
SMILES
O=C([C@@H](CCC/N=C(N)\N)N)NCC(N[C@H](C(N[C@@H](C(O)=O)CO)=O)CC(O)=O)=O
الصيغة الجزيئية
C15H27N7O8
الوزن الجزيئي
433.42
الذوبانية
≥ 21.65mg/mL in Water
التخزين
Desiccate at -20°C
إرشادات عامة
اختر مذيبًا مناسبًا وفقًا لبيانات ذوبانية المنتج. بعد التحضير، قسّم المحلول المخزون إلى أجزاء لتجنب دورات التجميد والذوبان المتكررة.
استخدم المحاليل المخزونة خلال 6 أشهر عند حفظها في -80°C، أو خلال شهر واحد عند حفظها في -20°C.
لتحسين الذوبانية، سخّن الأنبوب إلى 37°C وعرّضه للموجات فوق الصوتية لمدة مناسبة.
شروط الشحن
تُشحن عينات التقييم مع عبوات تبريد. وتُشحن الأحجام الأخرى المتاحة في درجة حرارة الغرفة، أو مع عبوات تبريد عند الطلب.

الحاسبات (Calculators)المولارية / التخفيف / الوزن الجزيئي / تحويل الوحدات / تركيبة داخل الجسم الحي / الذوبانية

g/mol

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