CAY10732 |
Catalog No.GC43206
One-Click Copy Product Info
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CAY10732 functions as a fluorescent agent designed to identify the presence of carbon monoxide.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 855751-82-5
Sample solution is provided at 25 µL, 10mM.
CAY10732 functions as a fluorescent agent designed to identify the presence of carbon monoxide.1 When exposed to palladium (Pd) and carbon monoxide, CAY10732 initiates the Pd0-mediated Tsuji-Trost reaction, resulting in the liberation of 2,7-dichlorofluorescein. The fluorescence emitted by this compound serves as an indicator of carbon monoxide production, both within controlled laboratory settings and within living cells. The excitation/emission peaks of 2,7-dichlorofluorescein are measured at 493/527 nm, respectively[1].
References:
[1]. Feng, S, Liu, D, et al. Allyl fluorescein ethers as promising fluorescent probes for carbon monoxide imaging in living cells. Anal. Chem. 89(6), 3754-3760 (2017).
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Protocol for Imaging of CO in Living Cells[1]： |
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Stock Solution Preparation：Stock solutions of CAY10732 (2 mM), PdCl2 (2 mM), and CORM-3 (10 mM) were separately prepared in HPLC grade DMSO and used freshly. CAY10732 (1 μM) and PdCl2 (1 μM) in PBS buffer (10 mM, pH 7.4, with 0.5% DMSO, v/v) was prepared in use. 1.HeLa cells were seeded in a 24-well culture plate for one night before cell imaging experiments. 2.In a typical experiment of cell imaging, as controls, living cells were incubated with CAY10732 (1 μM) and a mixture of CAY10732 and PdCl2 (1 μM each) at 37℃ for 30 min, respectively, and they were imaged after washing with PBS for three times. 3.For imaging of exogenous CO, HeLa cells were pretreated with CORM-3 (1, 5, and 10 μM, respectively) for 30 min at 37℃ and then were incubated with a mixture of CAY10732 and PdCl2 (1 μM each) for 30 min at 37℃. After washing the cells with PBS buffer, the cells were imaged. 4.For imaging of heme stimulation produced CO, HeLa cells were pretreated with heme (100 μM) for 30 min at 37℃ and then were incubated with a mixture of CAY10732 and PdCl2 (1 μM each) for 30 min at 37℃. After washing the cells with PBS buffer, the cells were imaged. This protocol only provides a guideline, and should be modified according to your specific needs. |
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References: [1]. Feng, S, Liu, D, et al. Allyl fluorescein ethers as promising fluorescent probes for carbon monoxide imaging in living cells. Anal. Chem. 89(6), 3754-3760 (2017). |
| Cas No. | 855751-82-5 | SDF | |
| Chemical Name | 3',6'-bis(2-propen-1-yloxy)-spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one | ||
| Canonical SMILES | O=C(C1=C2C=CC=C1)OC32C4=C(C=C(OCC=C)C=C4)OC5=C3C=CC(OCC=C)=C5 | ||
| Formula | C26H20O5 | M.Wt | 412.4 |
| Solubility | DMF: 12 mg/ml,DMSO: 10 mg/ml,Ethanol: 1 mg/ml | Storage | -20°C,protect from light. |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.4248 mL | 12.1242 mL | 24.2483 mL |
| 5 mM | 485 μL | 2.4248 mL | 4.8497 mL |
| 10 mM | 242.5 μL | 1.2124 mL | 2.4248 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 18 reference(s) in Google Scholar.)