GLPBio

Cholesterol esterase

Catalog No.: GC19870Purity:42 u/mg solid
COASDSData Sheet
Cholesterol esterase is a hydrolase that catalyzes the conversion of ingested cholesterol ester to free cholesterol and free fatty acid.

Products are for research use only. Not for human use. We do not sell to patients.

Cholesterol esterase
Cas No.: 9026-00-0
500UIn Stock
$45.00
1
1KUIn Stock
$75.00
1
5KUIn Stock
$300.00
1
Tel: (909) 407-4943 / (909) 689-7124Email: sales@glpbio.com

Citations

No citations are currently available for this product. Below are selected publications citing GlpBio products.
  • Nature cover
    Nature
    641, 529–536 (2025)
  • Nature cover
    Nature
    628, 630–638 (2024)
  • Nature cover
    Nature
    632, 686–694 (2024)
  • Nature cover
    Nature
    618, 1017–1023 (2023)
  • Nature cover
    Nature
    610, 366–372 (2022)
  • Cell cover
    Cell
    187(9):2288-2304 (2024)
  • Cell cover
    Cell
    183(7):1867-1883 (2020)
  • Science cover
    Science
    388(6745) (2025)
  • Science cover
    Science
    387(6739) (2025)
  • Science cover
    Science
    387(6734) (2025)
  • Cell Research cover
    Cell Research
    35, 97–116 (2025)
  • Cell Research cover
    Cell Research
    34, 683–706 (2024)
  • Cell Research cover
    Cell Research
    33, 273–287 (2023)
  • Cell Research cover
    Cell Research
    33, 546–561 (2023)
  • Cell Research cover
    Cell Research
    33, 904–922 (2023)
  • Cell Research cover
    Cell Research
    31, 1291–1307 (2021)

Description

Cholesterol esterase is a hydrolase that catalyzes the conversion of ingested cholesterol ester to free cholesterol and free fatty acid [1]. Cholesterol esterase exhibits proatherogenic properties by facilitating the conversion of the larger and less atherogenic lipoprotein particles to smaller and more atherogenic lipoprotein subspecies [2]. Cholesterol esterase can be covalently immobilized on the polyaniline film to develop a biosensor for cholesterol measurement[3].

In vitro, Cholesterol esterase (100mU/ml) treatment for 3 hours promotes the proliferation and migration of human umbilical vein endothelial cells (HUVECs) [4].

References:
[1] Heidrich J E, Contos L M, Hunsaker L A, et al. Inhibition of pancreatic cholesterol esterase reduces cholesterol absorption in the hamster[J]. BMC pharmacology, 2004, 4(1): 5.
[2] Li B, Zhou B, Lu H, et al. Phosphaisocoumarins as a new class of potent inhibitors for pancreatic cholesterol esterase[J]. European journal of medicinal chemistry, 2010, 45(5): 1955-1963.
[3] Singh S, Solanki P R, Pandey M K, et al. Covalent immobilization of cholesterol esterase and cholesterol oxidase on polyaniline films for application to cholesterol biosensor[J]. Analytica Chimica Acta, 2006, 568(1-2): 126-132.
[4] Rebaï O, Le Petit-Thevenin J, Bruneau N, et al. In vitro angiogenic effects of pancreatic bile salt-dependent lipase[J]. Arteriosclerosis, thrombosis, and vascular biology, 2005, 25(2): 359-364.

Protocol

Cell experiment [1]:

Cell lines

Human umbilical vein endothelial cells (HUVECs)

Preparation Method

HUVECs were incubated with EBM-2 medium supplemented with 2% fetal calf serum (FCS) in a humidified atmosphere at 37°C with 5% CO2. HUVEC migration was determined using a 24-well chemotaxicell chamber with polycarbonate filter (8-μm pore size). HUVECs (2×104 cells) in serum-free medium (100μl) were applied in the upper compartment of the chamber. The medium containing Cholesterol esterase (100mU/ml) was added to the lower compartment of the chamber and incubated (37°C; 3 hours). After removing Cholesterol esterase and a further incubation (37°C; 24 hours), the non-invading cells on the upper surface of the filter were completely wiped off with a cotton swab and filters were fixed (70% ethanol in PBS) and stained (hematoxylin). The number of migrated cells was counted in 10 random fields at 200× magnification under the microscope.

Reaction Conditions

100mU/ml; 3h

Applications

Cholesterol esterase treatment significantly promoted cell migration of HUVECs.

References:
[1] Rebaï O, Le Petit-Thevenin J, Bruneau N, et al. In vitro angiogenic effects of pancreatic bile salt-dependent lipase[J]. Arteriosclerosis, thrombosis, and vascular biology, 2005, 25(2): 359-364.

Product Documents

Biological Activity:42 u/mg solidAppearance:A solid
COASDSData Sheet

Chemical Properties

CAS No.
9026-00-0
Solubility
H2O : ≥ 50 mg/mL
Storage
Store at -20°C
General tips
Select a suitable solvent according to the product solubility data. Once prepared, aliquot the stock solution to avoid repeated freeze-thaw cycles.
Use stock solutions within 6 months when stored at -80°C or within 1 month when stored at -20°C.
To improve solubility, warm the tube to 37°C and sonicate it for an appropriate period.
Shipping Condition
Evaluation samples are shipped with blue ice. Other available sizes are shipped at room temperature or with blue ice upon request.

CalculatorsMolarity / Dilution / Molecular Weight / Unit Conversion

g/mol

Reviews

Review for Cholesterol esterase

Rating: 5
  • 5 Star100%
  • 4 Star0%
  • 3 Star0%
  • 2 Star0%
  • 1 Star0%