Cirsimaritin (Synonyms: 5,4'-Dihydroxy-6,7-dimethoxyflavone) |
Catalog No.GC31120
One-Click Copy Product Info
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Cirsimaritin is a dimethoxy flavonoid found in plants, it has anticancer potential and shows potent antimicrobial, antioxidant, antispasmodic, activity, and also inhibits cyclooxygenase-1.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 6601-62-3
Sample solution is provided at 25 µL, 10mM.
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Cirsimaritin is a dimethoxy flavonoid found in plants, it has anticancer potential and shows potent antimicrobial, antioxidant, antispasmodic, activity, and also inhibits cyclooxygenase-1[1-3].
Cirsimaritin significantly decreased cell proliferation in the NCIH-520 cell line, with an IC50 value of 23.29 ± 3.51µM[4]. Cirsimaritin(0–120µg/mL; 20h) inhibited interleukin-6, tumor necrosis factor-α, and NO production in a concentration-dependent manner in lipopolysaccharide (LPS)-stimulated RAW264.7 cells[5]. Cirsimaritin(5-20µg/mL) reduced viral titers and influenza A virus (IAV) RNA and protein synthesis in a dose-dependent manner[6].
Cirsimaritin(10mg/kg; i.g)ffectively prevented DSS-induced colon inflammation in mice by mitigating mucosal damage and inflammatory cell infiltration. It significantly downregulated proinflammatory cytokines such as MMP9, NF-κB, COX-2, and iNOS. Additionally, cirsimaritin reversed DSS-induced effects, including weight loss, colon shortening, tissue damage, increased inflammatory cells, and elevated cytokine secretion[7]. Treatment with cirsimaritin(0.5-1mg/kg; p.o; 8 weeks) reduced the severity of liver injury in the experimental mouse model of metabolic dysfunction-associated fatty liver disease (MAFLD) by inhibiting hepatic steatosis, oxidative stress, inflammation, and liver fibrosis[8].
References:
[1]. Benali T, Jaouadi I, et,al. The Current State of Knowledge in Biological Properties of Cirsimaritin. Antioxidants (Basel). 2022 Sep 19;11(9):1842. doi: 10.3390/antiox11091842. PMID: 36139916; PMCID: PMC9495358.
[2]. Ben Sghaier M, Skandrani I, et,al. Flavonoids and sesquiterpenes from Tecurium ramosissimum promote antiproliferation of human cancer cells and enhance antioxidant activity: a structure-activity relationship study. Environ Toxicol Pharmacol. 2011 Nov;32(3):336-48. doi: 10.1016/j.etap.2011.07.003. Epub 2011 Jul 12. PMID: 22004952.
[3]. Nyiligira E, Viljoen AM, et,al. Phytochemistry and in vitro pharmacological activities of South African Vitex (Verbenaceae) species. J Ethnopharmacol. 2008 Oct 28;119(3):680-5. doi: 10.1016/j.jep.2008.07.004. Epub 2008 Jul 16. PMID: 18678237.
[4]. Pathak G, Singh S, et,al. Cirsimaritin, a lung squamous carcinoma cells (NCIH-520) proliferation inhibitor. J Biomol Struct Dyn. 2021 Jun;39(9):3312-3323. doi: 10.1080/07391102.2020.1763198. Epub 2020 May 13. PMID: 32362196.
[5]. Shin MS, Park JY, et,al. Anti-inflammatory effects and corresponding mechanisms of cirsimaritin extracted from Cirsium japonicum var. maackii Maxim. Bioorg Med Chem Lett. 2017 Jul 15;27(14):3076-3080. doi: 10.1016/j.bmcl.2017.05.051. Epub 2017 May 17. PMID: 28554870.
[6]. sYan H, Wang H, et,al. Cirsimaritin inhibits influenza A virus replication by downregulating the NF-κB signal transduction pathway. Virol J. 2018 May 21;15(1):88. doi: 10.1186/s12985-018-0995-6. PMID: 29783993; PMCID: PMC5963025.
[7]. Alqudah A, Qnais E, et,al. Cirsimaritin Alleviates Dextran Sodium Sulfate-Induced Acute Colitis in Experimental Animals: A Therapeutic Approach for Inflammatory Bowel Disease. Prev Nutr Food Sci. 2024 Mar 31;29(1):31-39. doi: 10.3746/pnf.2024.29.1.31. PMID: 38576881; PMCID: PMC10987388.
[8]. Che DN, Shin JY, et,al. Ameliorative effects of Cirsium japonicum extract and main component cirsimaritin in mice model of high-fat diet-induced metabolic dysfunction-associated fatty liver disease. Food Sci Nutr. 2021 Sep 1;9(11):6060-6068. doi: 10.1002/fsn3.2548. PMID: 34760237; PMCID: PMC8565240.
| Cell experiment [1]: | |
Cell lines | NCIH-520 cell line |
Preparation Method | Cells were cultured in high glucose DMEM or RPMI media and supplemented with 1% Ab/Am and 10% FBS and placed in a CO2 incubator at 37 ℃ temperature in 95% humidity. Cells were seeded in a sterile 96-well flat-bottom cell culture plates with approximately 2 × 103 cells/well in each well, and the plates were incubated for 24h in CO2 incubator. After 24h of incubation, cells were treated with different concentrations of cirsimaritin, ranging from 0.1-100μM and followed by incubation for 24h. |
Reaction Conditions | 0.1-100μM; 24h |
Applications | Cirsimaritin significantly decreased cell proliferation in the NCIH-520 cell line, with an IC50 value of 23.29 ± 3.51µM. |
| Animal experiment [2]: | |
Animal models | Male Swiss albino mice |
Preparation Method | Mice were given a regimen of cirsimaritin, which was administered at a daily dose of 10mg/kg body weight (BW) through gavage, before they were exposed to DSS (4% DSS in their drinking water for 6 days). |
Dosage form | 10mg/kg; i.g |
Applications | Cirsimaritin maintains BW (body weight) and reduces DAI (disease activity index) in mice with DSS (dextran sulfate sodium)-induced colitis. |
References: | |
| Cas No. | 6601-62-3 | SDF | |
| Synonyms | 5,4'-Dihydroxy-6,7-dimethoxyflavone | ||
| Canonical SMILES | O=C1C=C(C2=CC=C(O)C=C2)OC3=CC(OC)=C(OC)C(O)=C13 | ||
| Formula | C17H14O6 | M.Wt | 314.29 |
| Solubility | DMSO : 22.73 mg/mL (72.32 mM; Need ultrasonic) | Storage | Store at -20°C,protect from light |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 3.1818 mL | 15.9089 mL | 31.8177 mL |
| 5 mM | 636.4 μL | 3.1818 mL | 6.3635 mL |
| 10 mM | 318.2 μL | 1.5909 mL | 3.1818 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 20 reference(s) in Google Scholar.)