Home>>Labeling & Detection>> Cyanine Dyes>>Cy3.5 hydrazide (non-sulfonated)

Cy3.5 hydrazide (non-sulfonated)

Catalog No.: GC13938

carbonyl-reactive dye

Cy3.5 hydrazide (non-sulfonated) Chemical Structure

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Sample solution is provided at 25 µL, 10mM.

Product Documents

Quality Control & SDS

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Cy3.5 hydrazide is a near infrared fluorophore that can react with various carbonyl groups in biomolecules which are generated after oxidative stress or deamination of proteins. In addition, aldehyde moieties can be produced by periodate oxidation of sugar residues of glycoproteins, such as antibodies that can then be oxidized by sodium periodate and labeled with Cy3.5 hydrazide to form Cy3. 5 labeled antibodies. For biomolecule labeling, the labeling regent has low aqueous solubility, using of organic co-solvent to dissolve this molecular is necessary for efficient reaction. First, Cyanine dye should be dissolved in organic solvent and then added to a solution of biomolecule in appropriate aqueous buffer.

Chemical Properties

Cas No. N/A SDF
Chemical Name 3-(6-hydrazinyl-6-oxohexyl)-1,1-dimethyl-2-((1E,3E)-3-(1,1,3-trimethyl-1H-benzo[e]indol-2(3H)-ylidene)prop-1-en-1-yl)-1H-benzo[e]indol-3-ium chloride hydrochloride
Canonical SMILES CN1C2=CC=C3C=CC=CC3=C2C(C)(C)/C1=C\C=C\C4=[N+](CCCCCC(NN)=O)C5=CC=C(C=CC=C6)C6=C5C4(C)C.Cl.[Cl-]
Formula C38H44Cl2N4O M.Wt 643.69
Solubility ≥ 64.4mg/mL in DMSO, ≥ 33.33 mg/mL in EtOH with ultrasonic Storage Store at -20°C
General tips For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.
Shipping Condition Evaluation sample solution : ship with blue ice
All other available size: ship with RT , or blue ice upon request

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.

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**When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / CoA (available online).


Spectral properties

Excitation max (nm) 591 Emission max (nm) 604
Extinction Coefficient (M-1cm-1) 116000 Quantum Yield 0.35
CF260 0.29 CF280 0.22


Review for Cy3.5 hydrazide (non-sulfonated)

Average Rating: 5 ★★★★★ (Based on Reviews and 37 reference(s) in Google Scholar.)

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