Cy3 hydrazide bromide |
Catalog No.GC80352
One-Click Copy Product Info
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Cy3 hydrazide bromide is a fluorescent probe for carbonylated proteins.
Products are for research use only. Not for human use. We do not sell to patients.
Sample solution is provided at 25 µL, 10mM.
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In Vitro, Cy3 hydrazide bromide (30 min (on ice labeling)) labels carbonylated proteins in mouse latissimus dorsi muscle extracts[1]. Cy3 hydrazide bromide (5 mM; 30 min) enables multiplexed detection of carbonylated proteins in hydrogen peroxide-treated Saccharomyces cerevisiae W303-1A cell extracts via two-dimensional gel electrophoresis, but with spot shifting that reduces alignment with total protein stain[2]. Cy3 hydrazide bromide (0.5-40 mM; 45 min-2 h) labels yeast tRNAPhe at dihydrouridine residues[3]. Cy3 hydrazide bromide (40 mM; 2 h) labels reduced Escherichia coli tRNAArg[3].
References:
[1]. Nina-Diogo A, et al. Synthesis of New Bodipy Hydrazide Fluorescent Probes for the Detection of Carbonylated Proteins Generated by Oxidative Stress. Chembiochem. 2024;25(19):e202400093.
[2]. Tamarit J, et al. Analysis of oxidative stress-induced protein carbonylation using fluorescent hydrazides. J Proteomics. 2012;75(12):3778-3788.
[3]. Pan D, et al. Synthesis and functional activity of tRNAs labeled with fluorescent hydrazides in the D-loop. RNA. 2009;15(2):346-354.
| Cas No. | SDF | ||
| Formula | C31H41BrN4O7S2 | M.Wt | 725.71 |
| Solubility | DMSO: 25 mg/mL (34.45 mM; Need ultrasonic and warming) | Storage | Store at 4°C, protect from light |
| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.378 mL | 6.8898 mL | 13.7796 mL |
| 5 mM | 275.6 μL | 1.378 mL | 2.7559 mL |
| 10 mM | 137.8 μL | 689 μL | 1.378 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.50% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)