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Cy5.5 hydrazide (non-sulfonated)

Catalog No.GC12343

carbonyl-reactive dye

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Cy5.5 hydrazide (non-sulfonated) Chemical Structure

Cas No.: N/A

Size Price Stock Qty
1mg
$154.00
In stock
5mg
$294.00
In stock
25mg
$574.00
In stock
50mg
$973.00
In stock
100mg
$1,660.00
In stock

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Sample solution is provided at 25 µL, 10mM.

Product Documents

Quality Control & SDS

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Background

Cy5.5 hydrazide is a reactive dye for the labeling of biomolecules containing carbonyl groups, such as ketone and aldehydes. This dye pocesses a hydrazide group that can be used to label glycans at their reducing end or other reducing sugars, forming a hydrazone linkage. Hydrazide-containing Cyanine Dyes can react with glycoproteins and other glycoconjugates specifically via their carbohydrate groups without interfering binding site or active centers. Glycoproteins also can be labeled after periodate oxidation to form aldehyde groups. Therefore, this dye is especially applicable for the labeling of antibody. Moreover, Cy5.5 is a near infrared fluorophore with prominent ability of tissue penetration, so it is suitable for the applications which require low fluorescence background, and for the in vivo NIR imaging. For biomolecule labeling, the labeling regent has low aqueous solubility, using of organic co-solvent to dissolve this molecular is necessary for efficient reaction. First, Cyanine dye should be dissolved in organic solvent and then added to a solution of biomolecule in appropriate aqueous buffer.

Chemical Properties

Cas No. N/A SDF
Chemical Name 3-(6-hydrazinyl-6-oxohexyl)-1,1-dimethyl-2-((1E,3E,5E)-5-(1,1,3-trimethyl-1H-benzo[e]indol-2(3H)-ylidene)penta-1,3-dien-1-yl)-1H-benzo[e]indol-3-ium chloride hydrochloride
Canonical SMILES CN1C2=CC=C3C=CC=CC3=C2C(C)(C)/C1=C\C=C\C=C\C4=[N+](CCCCCC(NN)=O)C5=CC=C(C=CC=C6)C6=C5C4(C)C.[Cl-].Cl
Formula C40H46Cl2N4O M.Wt 669.73
Solubility ≥ 67mg/mL in DMSO, ≥ 98.4 mg/mL in EtOH Storage Store at -20°C
General tips Please select the appropriate solvent to prepare the stock solution according to the solubility of the product in different solvents; once the solution is prepared, please store it in separate packages to avoid product failure caused by repeated freezing and thawing.Storage method and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored at -20°C, please use it within 1 month.
To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time.
Shipping Condition Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request.

Complete Stock Solution Preparation Table

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1 mg 5 mg 10 mg
1 mM 1.4931 mL 7.4657 mL 14.9314 mL
5 mM 0.2986 mL 1.4931 mL 2.9863 mL
10 mM 0.1493 mL 0.7466 mL 1.4931 mL
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.

Reviews

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Average Rating: 5 ★★★★★ (Based on Reviews and 4 reference(s) in Google Scholar.)

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