GLPBio

D-Allose

Catalog No.: GC62921Purity:>98.00%
COASDSData Sheet
D-Allose is an ultra-low-calorie aldohexose that exhibits cryoprotective function on cell survival during freezing.

Products are for research use only. Not for human use. We do not sell to patients.

D-Allose
Cas No.: 2595-97-3
100 mgIn Stock
$54.00
1
250 mgIn Stock
$108.00
1
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Description

D-Allose is an ultra-low-calorie aldohexose that exhibits cryoprotective function on cell survival during freezing [1]. D-Allose protects mouse hippocampal HT-22 cells from oxygen-glucose deprivation and reperfusion (OGD/R) -induced injury by inhibiting the Gal-3/TLR4 signalling pathway [2]. D-Allose has been widely used to inhibit the proliferation of a variety of cancer cells and prevent cancer cells from taking up glucose[3].

In vitro, D-Allose treatment for 48 hours significantly inhibited the viability of MIA PaCa-2 cells with an IC50 value of 53.25mM[4]. Treatment of cells with 40mM D-Allose for 48 hours inhibited the growth of PC-3 cells, induced apoptosis, and caused cell cycle arrest[5]. Treatment of RT112 cells with 50mM D-Allose for 24h inhibited cell viability, induced intracellular reactive oxygen species (ROS) production, and stimulated TXNIP expression[6].

In vivo, D-Allose treatment via intraperitoneal injection at a dose of 100mg/kg/day for 28 days significantly reduced tumor volume in a subcutaneous U87MG cell xenograft mouse model [7]. Intravenous administration of 0.4mg/g D-Allose 1 hour before ischemia significantly inhibited apoptosis, reduced blood brain barrier (BBB) permeability, and decreased inflammatory cytokine levels in the mice model of middle cerebral artery occlusion and reperfusion (MCAO/Rep) [8].

References:
[1] Chen Z, Chen J, Zhang W, et al. Recent research on the physiological functions, applications, and biotechnological production of D-allose[J]. Applied microbiology and biotechnology, 2018, 102(10): 4269-4278.
[2] Huang Y, Thonusin C, Tokuda M, et al. The beneficial effects of D-allose and D-allulose on the brain under ischemic stroke and obese-insulin resistant conditions: evidence from in vitro to clinical studies[J]. Metabolic Brain Disease, 2025, 40(4): 1-15.
[3] Noguchi C, Kamitori K, Hossain A, et al. D-allose inhibits cancer cell growth by reducing GLUT1 expression[J]. The Tohoku journal of experimental medicine, 2016, 238(2): 131-141.
[4] Malm S W, Hanke N T, Gill A, et al. The anti-tumor efficacy of 2-deoxyglucose and D-allose are enhanced with p38 inhibition in pancreatic and ovarian cell lines[J]. Journal of Experimental & Clinical Cancer Research, 2015, 34(1): 31.
[5] Naha N, Lee H Y, Jo M J, et al. Rare sugar D-allose induces programmed cell death in hormone refractory prostate cancer cells[J]. Apoptosis, 2008, 13(9): 1121-1134.
[6] Tohi Y, Taoka R, Zhang X, et al. Antitumor effects of orally administered rare sugar D-allose in bladder cancer[J]. International Journal of Molecular Sciences, 2022, 23(12): 6771.
[7] Suzuki K, Ogawa D, Kanda T, et al. Antiproliferative effects of D-allose associated with reduced cell division frequency in glioblastoma[J]. Scientific Reports, 2023, 13(1): 19515.
[8] Huang T, Gao D, Hei Y, et al. D-allose protects the blood brain barrier through PPARγ-mediated anti-inflammatory pathway in the mice model of ischemia reperfusion injury[J]. Brain research, 2016, 1642: 478-486.

Protocol

Cell experiment [1]:

Cell lines

 MIA PaCa-2 cells

Preparation Method

 MIA PaCa-2 cells were cultured in Dulbecco's Modified Eagle's medium (DMEM) supplemented with 10% fetal bovine serum and 4.5g/l glucose. Cells were seeded in 96-well plates at a density of 3000 cells/well, with the lateral wells left empty for water addition. Cells were treated with different concentrations of D-Allose (0.01, 0.1, 1,10, and 100mM) for 48 hours, and cell proliferation was analyzed.

Reaction Conditions

 0.01, 0.1, 1,10, and 100mM; 48h

Applications

 D-Allose treatment decreased the cell viability of MIA PaCa-2 cells in a dose-dependent manner.
Animal experiment [2]:

Animal models

 Male BALB/c (nu/nu) nude mice

Preparation Method

 Male BALB/c (nu/nu) nude mice, 5 weeks old and weighing 20g, were housed in a specific pathogen clearance grade (SPF) animal house at 24±2°C temperature and 55±5% humidity and maintained on a 12h light / 12h dark circadian rhythm. Mice received a standard diet and water AD libitum. U87MG cells (5×106 cells/100µl) were injected subcutaneously into the right abdomen of mice. When the tumor volume reached about 150mm3, the mice were randomly divided into two groups: the experimental group received D-Allose (100mg/kg; i.p.) once a day for 30 consecutive days; mice in the control group were intraperitoneally injected with normal saline. Tumor volume and body weight of mice were analyzed.

Dosage form

 100mg/kg/day for 30 days; i.p.

Applications

 D-Allose treatment suppressed tumor growth in the U87MG cell xenograft mouse models without affecting body weight.

References:
[1] Malm S W, Hanke N T, Gill A, et al. The anti-tumor efficacy of 2-deoxyglucose and D-allose are enhanced with p38 inhibition in pancreatic and ovarian cell lines[J]. Journal of Experimental & Clinical Cancer Research, 2015, 34(1): 31.
[2] Suzuki K, Ogawa D, Kanda T, et al. Antiproliferative effects of D-allose associated with reduced cell division frequency in glioblastoma[J]. Scientific Reports, 2023, 13(1): 19515.

Product Documents

Purity:>98.00%Appearance:A solid
COASDSData Sheet

Chemical Properties

CAS No.
2595-97-3
Molecular Formula
C6H12O6
Molecular Weight
180.16
Solubility
DMSO : 100 mg/mL (555.06 mM; Need ultrasonic); H2O : 100 mg/mL (555.06 mM; Need ultrasonic)
Storage
Store at 2-8°C,protect from light
General tips
Select a suitable solvent according to the product solubility data. Once prepared, aliquot the stock solution to avoid repeated freeze-thaw cycles.
Use stock solutions within 6 months when stored at -80°C or within 1 month when stored at -20°C.
To improve solubility, warm the tube to 37°C and sonicate it for an appropriate period.
Shipping Condition
Evaluation samples are shipped with blue ice. Other available sizes are shipped at room temperature or with blue ice upon request.

CalculatorsMolarity / Dilution / Molecular Weight / Unit Conversion / In Vivo Formulation / Solubility

g/mol

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