3-Hydroxyindole

3-Hydroxyindole is a hydroxyindole compound. 3-Hydroxyindole can be isolated from the fruits of Aristotelia chilensis. 3-Hydroxyindole inhibits Ferroptosis and scavenges DPPH free radicals. 3-Hydroxyindole protects against β-amyloid -induced toxicity. 3-Hydroxyindole exhibits antioxidant activity. 3-Hydroxyindole can be used in research related to neurodegenerative diseases ^{[1] [2] [3]}.

In Vitro, 3-Hydroxyindole potently scavenges DPPH radicals in a cell-free system with an IC₅₀ of 0.87 ppm, showing greater activity than α-tocopherol^[1]. 3-Hydroxyindole potently scavenges crocin radicals in a cell-free system with an IC₅₀ of 0.67 ppm^[1]. 3-Hydroxyindole potently inhibits lipid peroxidation in brain homogenate ex vivo with an IC₅₀ of 1.29 ppm^[1]. 3-Hydroxyindole (20 μM; 22-24 h) potently inhibits ferroptosis induced by Erastin,RSL3, and FINO2 in HT-22 mouse hippocampal neurons, with EC50 values of 7.9 μM, 3.5 μM, and 1.6 μM respectively^[2]. 3-Hydroxyindole (25 μM; 24 h) does not protect HT-22 mouse hippocampal neurons from non-ferroptotic toxicity induced by Rotenone, indicating its protective effects are specific to ferroptosis^[2]. 3-Hydroxyindole (24 h) potently inhibits ferroptosis induced by RSL3 and FINO2 in N27 rat dopaminergic neurons, with EC50 values of 1.5 μM and 2.2 μM respectively^[2].

References:
[1]. Céspedes CL, et al. Antioxidant activity of an unusual 3-hydroxyindole derivative isolated from fruits of Aristotelia chilensis (Molina) Stuntz. Zeitschrift fur Naturforschung. C, Journal of biosciences. 2009;64(9-10):759-62.
[2]. Jakaria M, et al. The role of hydroxyindoles in protecting neuronal cultures from ferroptosis. Cell death discovery. 2025 Jul 16;11(1):329.
[3]. Jakaria M, et al. Inhibition of Ferroptotic Toxicity by 4-Hydroxyindole. Chemical research in toxicology. 2025 Nov 17;38(11):2002-2009.