9(10)-Nitrooleate (Synonyms: 9(10)-Nitrooleic Acid; OA-NO2; 9(10)-nitro-9-trans-Octadecenoic Acid) |
Katalog-Nr.GC92043
One-Click Copy Product Info
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9(10)-Nitrooleate ist ein Gemisch der endogenen Lipidsignalmoleküle 9-nitrooleat.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 1092676-99-7
Sample solution is provided at 25 µL, 10mM.
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9(10)-Nitrooleate is a mixture of the nitroalkene endogenous lipid signaling molecules 9-nitrooleate. 9(10)-Nitrooleate is formed by nitration of oleic acid by peroxynitrite, acidified nitrite, and myeloperoxidase in the presence of hydrogen peroxide (H2O2) and nitrite.1 It induces transactivation of peroxisome proliferator-activated receptor γ (PPARγ) or nuclear factor erythroid 2-related factor 2 (Nrf2) in reporter assays using MCF-7 cells when used at concentrations greater than or equal to 0.3 and greater than or equal to 3 ?M, respectively.2 9(10)-Nitrooleate also induces transactivation of PPARγ, PPARδ, or PPARα in reporter assays using CV-1 cells in a concentration-dependent manner.1 It induces adipogenesis in 3T3-L1 preadipocytes and glucose uptake in differentiated 3T3-L1 adipocytes when used at a concentration of 3 ?M. In vivo, 9(10)-nitrooleate (500 ?g/kg every six hours for 24 hours) decreases ischemia-induced increases in plasma levels of blood urea nitrogen, creatinine, and TNF-α and kidney levels of myeloperoxidase (Mpo) and IL-1β and reduces tubular necrosis and tubule dilation in a mouse model of renal ischemia-reperfusion injury.3
References:
[1]. Baker, P.R., Lin, Y., Schopfer, F.J., et al.Fatty acid transduction of nitric oxide signaling. Multiple nitrated unsaturated acid derivatives exist in human blood and urine serve as endogenous peroxisome proliferator-activated receptor ligandsJ. Biol. Chem.280(51)42464-42475(2005).
[2]. Bates, D.J.P., Smitherman, P.K., Townsend, A.L., et al.Nitroalkene fatty acids mediate activation of Nrf2/ARE-dependent and PPARγ-dependent transcription by distinct signaling pathways and with significantly different potenciesBiochemistry50(36)7765-7773(2011).
[3]. Liu, H., Jia, Z., Soodvilai, S., et al.Nitro-oleic acid protects the mouse kidney from ischemia and reperfusion injuryAm. J. Physiol. Renal. Physiol.295(4)F942-F949(2008).
| Cas No. | 1092676-99-7 | SDF | |
| Überlieferungen | 9(10)-Nitrooleic Acid; OA-NO2; 9(10)-nitro-9-trans-Octadecenoic Acid | ||
| Chemical Name | 9-nitro-9E-octadecenoic acid, 10-nitro-9E-octadecenoate | ||
| Canonical SMILES | CCCCCCCC/C=C([N+]([O-])=O)\CCCCCCCC(O)=O.CCCCCCCC/C([N+]([O-])=O)=C\CCCCCCCC(O)=O | ||
| Formula | C18H33NO4 ? C18H33NO4 | M.Wt | 654.9 |
| Löslichkeit | Ethanol: Slightly soluble: 0.1-1 mg/ml | Storage | -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.527 mL | 7.6348 mL | 15.2695 mL |
| 5 mM | 305.4 μL | 1.527 mL | 3.0539 mL |
| 10 mM | 152.7 μL | 763.5 μL | 1.527 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >95.00% Appearance: A solution in ethanol
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)