Bromfenac Sodium

Cell lines

Corneal epithelial cells

Preparation method

The solubility of this compound in DMSO is > 14.7 mg/mL. General tips for obtaining a higher concentration: Please warm the tube at 37 °C for 10 minutes and/or shake it in the ultrasonic bath for a while. Stock solution can be stored below - 20 °C for several months.

Reacting condition

1, 4, 12, 24 and 48 hrs

Applications

Compared with Bromfenac Sodium, the cellular metabolic activity of Diclofenac and Fluorometholone markedly decreased after 12-hr exposure. However, the K+ and Cl- concentrations, pH and osmolarity were similar among different treatment groups. In additioin, Bromfenac Sodium significantly promoted cell migration, as well as restored wound gap after 48-hr exposure, compared with Diclofenac and Fluorometholone.

Animal models

A rabbit model of ocular inflammation

Dosage form

50 μL 0.09%

Applications

In a rabbit model of ocular inflammation, Bromfenac Sodium almost completely inhibited lipopolysaccharide (LPS)-induced increases in fluorescein isothiocyanate (FITC)-dextran in the anterior chamber as well as the contralateral eye. In addition, Bromfenac Sodium significantly inhibited LPS-induced increases in PGE2 concentrations in the aqueous humor.

Other notes

Please test the solubility of all compounds indoor, and the actual solubility may slightly differ with the theoretical value. This is caused by an experimental system error and it is normal.

References:

[1]. Lee JS, Kim YH, Park YM. The Toxicity of Nonsteroidal Anti-inflammatory Eye Drops against Human Corneal Epithelial Cells in Vitro. J Korean Med Sci. 2015 Dec;30(12):1856-64.

[2]. Waterbury LD, Silliman D, Jolas T. Comparison of cyclooxygenase inhibitory activity and ocular anti-inflammatory effects of ketorolac tromethamine and bromfenac sodium. Curr Med Res Opin. 2006 Jun;22(6):1133-40.