CYT296 is a target chromatin de-condensation compound. CYT296 can improve the induction of induced pluripotent stem cell (iPSCs) mediated by defined factors (OSKM) and induce an open chromatin state in Mouse Embryonic Fibroblast (MEFs) to facilitate somatic cell reprogramming. CYT296 can be used for cell replacement therapies and drug screening research.
CYT296 (250nM, 72 h) changes the MEF chromatin and allows easier access of Yamanaka factors and facilitates the reprogramming process[1].
CYT296 (250nM, 72 h) block heterochromatin assembly of MEFs to improve the generation of iPSC[1].
CYT296 (10-15 4F- and 2F -iPSC clones for subcutaneous injection, once) generates the iPSCs are pluripotent in the NOD-SCID mice[1].
References:
[1]. Wei X, et al. Small molecule compound induces chromatin de-condensation and facilitates induced pluripotent stem cell generation. J Mol Cell Biol. 2014 Oct;6(5):409-20.