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ddTTP

Katalog-Nr.GC64020 Copy One-Click Copy Product Info

ddTTP ist eines der 2',3'-Didesoxyribonukleosid-5'-Triphosphate (ddNTPs), das als kettenverlÄngernder Inhibitor der DNA-Polymerase fÜr die DNA-Sequenzierung fungiert.

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ddTTP Chemische Struktur

Cas No.: 611-60-9

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1µmole
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5µmole
139,00 $
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10µmole
252,00 $
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Sample solution is provided at 25 µL, 10mM.



Description of ddTTP

ddTTP is one of 2’,3’-dideoxyribonucleoside 5’-triphosphates (ddNTPs) that acts as chain-elongating inhibitor of DNA polymerase for DNA sequencing[1].

[1]. Z G Chidgeavadze, et al. 3’-Fluoro-2’,3’-dideoxyribonucleoside 5’-triphosphates: terminators of DNA synthesis. FEBS Lett. 1985 Apr 22;183(2):275-8.

Chemical Properties of ddTTP

Cas No. 611-60-9 SDF
Formula C10H17N2O13P3 M.Wt 466.17
Löslichkeit Storage Store at -20°C
General tips Please select the appropriate solvent to prepare the stock solution according to the solubility of the product in different solvents; once the solution is prepared, please store it in separate packages to avoid product failure caused by repeated freezing and thawing.Storage method and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored at -20°C, please use it within 1 month.
To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time.
Shipping Condition Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request.

Complete Stock Solution Preparation Table of ddTTP

Prepare stock solution
1 mg 5 mg 10 mg
1 mM 2.1451 mL 10.7257 mL 21.4514 mL
5 mM 429 μL 2.1451 mL 4.2903 mL
10 mM 214.5 μL 1.0726 mL 2.1451 mL
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Berechnen

In vivo Formulation Calculator (Clear solution) of ddTTP

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % saline
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Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.

Product Documents

Quality Control & SDS

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Average Rating: 5 ★★★★★ (Based on Reviews and 18 reference(s) in Google Scholar.)

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