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Polybrene

Katalog-Nr.GC19206

Polybrene (Polybrene) ist ein kationisches Polymer, das routinemäßig zur Verbesserung der Effizienz der retroviralen Vektor-vermittelten Genübertragung eingesetzt wird.

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Polybrene Chemische Struktur

Cas No.: 28728-55-4

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Sample solution is provided at 25 µL, 10mM.

Product has been cited by 3 publications

Product Documents

Quality Control & SDS

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Protocol

Cell experiment [1]:

Cell lines

Induced pluripotent stem cell (iPSC)-differentiated neuron system

Reaction Conditions

0-32 µg/mL;24h

Applications

Polybrene displays concentration-dependent effects. Starting at concentrations as low as 4 µg/mL, distinct neuritic beads emerge, whereas a concentration of 8 µg/mL induces neuritic fragmentation.

Animal experiment [2]:

Animal models

Adult (2-3 months) BALB/c mice

Preparation Method

4 µg/µL polybrene was incubated at 37℃ for 15 min, and intracerebroventricularly (ICV) injection of 5 µL mixture was performed for 20 min. Rotating Rod test will take place in three days.

Dosage form

4 µg/µL;ICV

Applications

Polybrene injection resulted in mice exhibiting motor dysfunction on the third day.

References:

[1]. Bao F, Shi H,et,al. Polybrene induces neural degeneration by bidirectional Ca2+ influx-dependent mitochondrial and ER-mitochondrial dynamics. Cell Death Dis. 2018 Sep 20;9(10):966. doi: 10.1038/s41419-018-1009-8. PMID: 30237514; PMCID: PMC6148003.

Background

Polybrene, ein quaternäres Ammoniumsalz, wurde zunächst in der klinischen Praxis als Heparin-Neutralisator eingeführt[1]. Darüber hinaus wurde Polybrene in niedrigeren Dosen häufig verwendet, um die Effizienz der virusvermittelten Genübertragung sowohl in vivo als auch in vitro zu fördern[2-5]. Polybrene fördert die Bindung von Viren an der Zelloberfläche durch Neutralisierung der elektrostatischen Abstoßung zwischen den gegenüberliegenden Bilayern[6-7].

Polybrene (0-32 µg/mL; 24h) zeigt konzentrationsabhängige Effekte. Bereits bei Konzentrationen von nur 4 µg/mL bilden sich deutliche neuritische Perlen, während eine Konzentration von 8 µg/mL zur Fragmentierung der Neuriten führt[8]. Polyethylen (8µg/mL) reduziert signifikant die Proliferationsrate mesenchymaler Stammzellen (MSCs)[9]. Polybrene verbessert die Effizienz der Lipofektion erheblich und kompensiert auch die serumvermittelte Hemmung der Lipofektion in einer Vielzahl von Zelllinien[10].

Die Injektion von Polybrene (4 µg/µL; ICV) führte bei Mäusen am dritten Tag zu motorischen Störungen. Polybrene verursacht neuronales Sterben und Gliose bei Mäusen[8].

References:
[1]. Weiss.W. A, Gilman, J. S, et,al. Heparin neutralization with polybrene administered intravenously. J. Am. Med. Assoc. 166, 603-607 (1958).
[2]. Davis, H. E., Morgan, J. R. et,al. Polybrene increases retrovirus gene transfer efficiency by enhancing receptor-independent virus adsorption on target cell membranes. Biophys. Chem. 97, 159-172 (2002).
[3]. Bellodi-Privato, M. et al. AAV gene transfer to the retina does not protect retrovirally transduced hepatocytes from the immune response. J. Mol. Med (Berl.). 82, 403-410 (2004).
[4]. Lauwers, E. et al. Neuropathology and neurodegeneration in rodent brain induced by lentiviral vector-mediated overexpression of alpha-synuclein. Brain Pathol. 13, 364-372 (2003).
[5]. Ferry, N., Duplessis, O., et,al. Retroviral-mediated gene transfer into hepatocytes in vivo. Proc. Natl Acad. Sci. USA 88, 8377-8381 (1991).
[6]. Sinn, P. L., Sauter, S. L. et,al. Gene therapy progress and prospects: development of improved lentiviral and retroviral vectors--design, biosafety, and production. Gene Ther. 12, 1089-1098 (2005).
[7]. Kafri, T. Lentivirus vectors: difficulties and hopes before clinical trials. Curr. Opin. Mol. Ther. 3, 316-326 (2001).
[8]. Bao F, Shi H, et,al. Polybrene induces neural degeneration by bidirectional Ca2+ influx-dependent mitochondrial and ER-mitochondrial dynamics. Cell Death Dis. 2018 Sep 20;9(10):966. doi: 10.1038/s41419-018-1009-8. PMID: 30237514; PMCID: PMC6148003.
[9].Lin P, Correa D, et,al. Polybrene inhibits human mesenchymal stem cell proliferation during lentiviral transduction. PLoS One. 2011;6(8):e23891. doi: 10.1371/journal.pone.0023891. Epub 2011 Aug 26. PMID: 21887340; PMCID: PMC3162604.
[10].Abe A, Miyanohara A, Friedmann T. Polybrene increases the efficiency of gene transfer by lipofection. Gene Ther. 1998 May;5(5):708-11. doi: 10.1038/sj.gt.3300652. PMID: 9797877.

Chemical Properties

Cas No. 28728-55-4 SDF
Formula M.Wt
Löslichkeit 10mg/ml in Water Storage Store at -20℃, stored under nitrogen
General tips Please select the appropriate solvent to prepare the stock solution according to the solubility of the product in different solvents; once the solution is prepared, please store it in separate packages to avoid product failure caused by repeated freezing and thawing.Storage method and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored at -20°C, please use it within 1 month.
To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time.
Shipping Condition Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request.
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Related Video

    Polybrene - GlpBio

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