Glucose oxidase(100-250u/mg)

Glucose oxidase(100-250u/mg) is an oxidoreductase derived from Aspergillus niger. When oxygen and glucose are present, Glucose oxidase (100-250u/mg) can exhibit antibacterial activity ^{[1] [2] [3]}.

Glucose oxidase (100-250u/mg) is a yellow amorphous powder derived from Aspergillus niger. Its enzyme commission number is EC 1.1.3.4. Under specific conditions (pH 5.7, 37°C), one unit of glucose oxidase can oxidize 1μM glucose per minute. The molecular weight of the enzyme is about 150 kDa as determined by SDS-PAGE. Its isoelectric point is 5.2, showing certain charge properties. For the substrate D-glucose, the Michaelis constant of the enzyme is 4.9×10^-2 M. Actual measurements show that the pH stability of the product is 5.5~7.5 (25°C, 18 hours) and the thermal stability is <40°C (pH 5.7, 60 minutes). It is worth noting that metal ions such as Cu²⁺, Fe³⁺, Mg²⁺ and Mn²⁺ have an inhibitory effect on glucose oxidase (100-250u/mg). Glucose oxidase(100-250u/mg) specifically binds to β-D-pyranose glucose and does not act on α-D-glucose. In the presence of air and glucose, Glucose oxidase(100-250u/mg) catalyzes the oxidation of β-D-glucose to D-glucono-1,5-lactone, which is then hydrolyzed to gluconic acid^[4]. Glucose oxidase(100-250u/mg) is widely used in blood glucose monitoring, free glucose detection in plant raw materials and food industries, and food preservation. It also has many applications in biotechnology, usually for enzyme assays in biochemistry, biosensors in nanotechnology^[5].

References:
[1].Wong C M, Wong K H, Chen X D. Glucose oxidase: natural occurrence, function, properties and industrial applications[J]. Applied microbiology and biotechnology, 2008, 78: 927-938.
[2].Broom W A, Coulthard C E, Gurd M R, et al. Some pharmacological and chemotherapeutic properties of notatin[J]. British journal of pharmacology and chemotherapy, 1946, 1(4): 225.
 [3].Ghoshdastider U, Wu R, Trzaskowski B, et al. Molecular effects of encapsulation of glucose oxidase dimer by graphene[J]. RSC Advances, 2015, 5(18): 13570-13578.
[4]. Bauer J A, Zámocká M, Majtán J, et al. Glucose oxidase, an enzyme “Ferrari”: Its structure, function, production and properties in the light of various industrial and biotechnological applications[J]. Biomolecules, 2022, 12(3): 472.
[5].Heller A, Ulstrup J. Detlev Müller’s Discovery of Glucose Oxidase in 1925[J]. Analytical Chemistry, 2021, 93(18): 7148-7149.